Page 50 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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TOCOPHEROLS IN THE FRUITS OF ALTAI SEA BUCKTHORN CULTIVARS
Inessa Ershova

Federal Altai Scientific Centre of Agro-Biotechnologies, Barnaul, Russian Federation;
E-mail.: inessers@yandex.ru

Tocopherols (Vitamin E) are characterized by unique biological properties and high
antioxidant activity. The fruits of sea buckthorn (Hippophaё rhamnoides L.) serve as a highly
valuable source of natural Vitamin E, thus, it is important to study tocopherol content in the
fruits of this crop, aiming to identify and create new cultivars and varieties with high content of
this type of biologically active substances. Altai cultivars of sea buckthorn have high potential
in this regard.
The present research aims to study the content of tocopherols in the fruits of nine sea
buckthorn cultivars with high economic and biochemical potential: Chuiskaya, Elizaveta, Zlata,
Inya, Ognivo, Chechek, Chulishmanka, Essel, and Ethna. The material of the research consisted
of freshly harvested fruits, frozen in the freezer at -25 С. The extraction of tocopherols was
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performed by the modified method equivalent to the I.K. Murry method of extraction of
carotenoids. Ethanol 96 % and benzol were used as extractants. The sample weight was 5 g. 10
ml of finite benzol fraction was a vacuum- concentrated by 20 times at the temperature of 35 С
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and used for chromatographic analysis. The chromatography was performed with liquid
chromatograph Agilent LC 1260 Infinity, using column Zorbax Eclipse Plus C18, size 2.1*50 mm,
sorbent grain – 1.8 microns. The conditions of chromatography were: sample volume – 0.25–
0.5 microliters, mobile phase – water-acetonitrile (5 % Н2О, 95 % ACN), flow rate – 0.2 ml/min,
time – 30 minutes. Mass spectrums of the samples were registered by mass spectrometer
Agilent 6530 QTOF. Quantitative analysis was based on the analysis of MRM transition
430,3795→165,0912, the voltage of collision cell being 20 V in positive ionization mode. A
calibration curve was built on a standard sample of Vitamin E («Aldrich»).
The research shows a rather high level of tocopherol content that, however, differs
substantially in the fruits of the abovementioned cultivars of sea buckthorn. The average
tocopherol quantity is 96.1 mg/100 g, with a variation range 71.8–126.0 mg/100 g in wet
weight. Cultivar Chuiskaya , known for the high content of biologically active substances in its
fruits, also takes one of the leading positions in this research. The quantity of Vitamin E in this
variety is 116.9 mg/100 g, which is higher than average in other varieties and very substantial
for this crop. Red-fruited cultivar Ognivo has the highest tocopherol content: 126.0 mg/100 g.
The enhanced content of Vitamin E is also remarked in cultivar Chechek (99.7 mg/100 g) and
sweet-fruited cultivar Essel (92.9 mg/100 g). The rest of the cultivars, according to the
literature data, are also considered high in Vitamins. The corresponding variation range for
them is 71.8–88.4 mg/100 g. In this group cultivar Chulishmanka deserves attention, as it has
the highest content of tocopherols in the fruits – 88.4 mg/100 g, and, like cultivar Chyiskaya, it
is characterized by the high content of biologically active substances. The high content of
Vitamin E is remarked in red-fruited cultivars and forms of sea buckthorn.

Keywords: sea buckthorn, cultivars, fruits, tocopherols.

5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and |49
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Spiritual Human Development
November 3 2021
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