Page 48 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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EFFECT OF ALGINITE ON SUBSTANCES OF GENUS MENTHA REPRESENTATIVES AND
THEIR TOXICITY
Jarmila Eftimová, Vladimír Petrovič, Vladimír Vodhanel
Department of Pharmaceutical Technology, Pharmacognosy and Botany, University of Veterinary
Medicine and Pharmacy in Košice, Slovak Republic; E-mail.: jarmila.eftimova@uvlf.sk
The experimental work aimed to compare the influence of different application forms of
alginite on the amounts of substances in Mentha species "Erdbeerminze" and Mentha species
"Schokominze" and their toxicity on freshwater invertebrates of the genus Daphnia spp.
We determined the content of total polyphenols, phenolic acids, and antioxidant activity
by spectrophotometric methods. The highest content of total polyphenols converted to coffee
acid equivalent was determined in the aqueous extract of a sample of Mentha species
"Schokominze" in the control variant 3.37 mg CAE/100 g of dry drug. The lowest in the sample
of Mentha species "Erdbeerminze" variant powdered alginite, 1.01 mg CAE/100g of the dried
drug had the statistically lowest content.
The highest antioxidant activity determined by the method with DPPH radical was
observed in the methanol extract of Mentha species "Schokominze" in the variant with powdered
alginite 40.52 % and in the dressing with alginite 39.87 %. The differences between the alginite
and control variants are statistically insignificant. The highest antiradical activity (as Trolox
equivalent) was shown by the methanol extract of Mentha "Schokominze" variant alginite
powder, namely 119.5 mg TEAC/100 g of dry drug.
The content of phenolic acids in the ethanol extracts of the sample Mentha species
"Schokomize" was determined in the range from 1.52–1.57 mg CAE/100 g of dried drug. No
statistical differences in phenolic acid content were observed between variants within one
species.
Results of a 48-hour ecotoxicological experiment on Daphnia sp. indicate that the contents
of the extracts from the sample of Mentha species "Schokominze" had approximately 10 times
higher EC50 values than in all samples of Mentha species "Erdbeerminze", regardless of the form
of alginite application. The results showed that the extract from Mentha species "Schokominze"
in the control variant was for invertebrates Daphnia sp. the most toxic.
Keywords: Mentha, essential oil, menthol, Erdbeerminze, Schokominze, alginite.

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5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and |47
Spiritual Human Development
November 3 2021
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