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5 International Scientific Online Conference   DOI: https://doi.org/10.15414/2021.9788055224015

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                  FORMATION AND PRESERVATION OF COLLECTIONS OF GENETIC RESOURCES OF
                                             LEGUMES AND GROATS CROPS
                      Oleh Tryhub, Serhiy Sylenko, Viktoria Voroncova, Olena Andruschenko,
                                                   Oleksandr Rohovyi
                     Ustymivska Experimental Station of Plant Production of Plant Production Institute
                    n.a. V.Y. Yuryev NAAS of Ukraine, Ustymivka, Ukraine; E-mail.: trygub_oleg@ukr.net
                  Global processes triggered by human activity and natural changes of flora are destroying
             thousands  of  species  each  year.  Throughout  evolution,  some  of  those  species  (including
             legumes and groats crops) have formed the human food resources foundation on the planet.
             Natural reserves containing wild species of cultivated plants are becoming extinct, the situation
             with local varieties and forms is equally daunting. The samples of these species have been
             collected  for  centuries,  and  they  are  the  carriers  of  unique  quality  indicators  of  extreme
             importance for human health and the backbone of a huge polymorphism based on economic
             and  selection  value  characteristics.  Selection  institutions  are  solving  the  global  task  of
             increasing the productivity and quality of new varieties and hybrids. That it can only be solved
             by  involving  a  new  source  material  with  a  combination  of  relevant  parameters,  including
             resistance to abiotic environmental factors, disease, and pest damage in the selection process.
                  Collections  are  the  reserves  of  such  material.  In  Ukraine,  one  of  the  institutions
             specializing  in  the  formation  and  preservation  of  genetic  resources  is  the  Ustymivska
             Experimental Station of Plant Production, a part of the System of Genetic Resources of Plants of
             Ukraine.  During  over  60  years  of  work  on  mobilization,  study,  and  preservation  of  plant
             resources, the institution has accumulated a gene pool of legumes and groat crops totaling more
             than 13.3 thousand samples (including 5886 millet, 3022 beans, 1643 buckwheat, 1384 grass
             pea, and 1391 vetches, lupines and vignes). The collections are gathered using annual large-
             scale  expeditionary  meetings  on  the  territory  of  Ukraine  and  other  countries,  a  wide
             omnidirectional  exchange  of  material  with  the  world's  leading  gene  banks,  selection
             institutions, and research centers, providing mutually beneficial exchange, aimed to enrich the
             collections. The full-fledged collection of samples is facilitated by the extraordinary natural and
             climatic  diversity  of  Ukrainian  territory  that  has  formed  a  dramatically  diverse  flora
             representation – from steppe and semidesert climate to Polissya, submontane, and mountain
             with excessive rainfall. Each of the samples of the collection is represented in an electronic
             database from the moment the seeds are included in the collection until the fact of its transfer
             to plant breeders for work or other institutions for research.
                  The experimental station stores the collection material according to the type of medium-
             term  ex-situ  storage  in  compliance  with  international  standards  of  seed  preparation  and
             storage. Natural and climatic conditions of the research station’s location (transition zone from
             the Forest-Steppe to the Steppe) ensure the completeness of reproduction of newly obtained
             samples and obtaining quality seeds. Direct storage is carried out in a chamber with controlled
             conditions – dry air at a temperature of + 2–4  C. The number of seeds stored is sufficient to
                                                             o
             meet the needs of selection institutions, and when needed (i.e. in case of a significant order,
             reduction of grain, or reduced germination in comparison with optimal parameters) scientific
             units propagate it and put it into storage again.
                  Aiming to preserve the seeds in case of natural disasters and cataclysms, to be able to
             restore  unique  species  of  cultivated  plants  that  will  disappear  forever  from  the  earth's
             biosphere, the Ustymivska Experimental Station of Plant Production transferred 231 samples
             of the grass pea (Lathyrus sativus L.) into in World Seed Vault in the Svalbard archipelago.
            Keywords: collections, legumes and groats crops, ex-situ conservation.





             5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and  |138
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                                               Spiritual Human Development
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