Page 143 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015
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DYNAMIC OF POLYPHENOL COMPOUND CONTENT OF SYMPHYTUM SPP.
2
1
Olena Vergun , Eva Ivanišová , Yulia Vinogradova , Olga Shelepova , Ján Brindza
2
3
3
1 M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine,
Kyiv, Ukraine; E-mail.: olenavergun8003@gmail.com
2 Slovak Agricultural University in Nitra, Nitra, Slovakia
3 Tsitsin Main Botanical Garden, Russian Academy of Sciences, Moscow, Russian Federation
Plants from the Symphytum L. genus (Boraginaceae Juss.) are perennial and well-known
as medicinal from ancient times. The therapeutic effect of these plants is based on the content
of biologically active compounds, among which are polyphenols. Polyphenol compounds are
secondary metabolites that protect plant organisms from UV radiation, pathogens, oxidative
stress and are known as strong antioxidants. The search for new sources of polyphenols from
plant raw materials has continued last decades. Plants from natural flora are not inferior to
cultural species in the content of different groups of polyphenol compounds.
This study aimed to determine the content of groups of polyphenol compounds in
Symphytum spp. extracts during vegetation. In the experiment used plant raw Symphytum
asperum Lepech., S. caucasicum Bieb., and S. ×uplandicum Nyman collected at the spring
vegetation (regrowth), stem growing, budding, flowering, and fruitage during 2019–2020.
Plants took from the natural flora of M.M. Gryshko National Botanical Garden of the NAS of
Ukraine. All investigations were conducted in the Slovak University of Agriculture in Nitra
(Slovak Republic). Ethanol extracts of investigated plants were measured for total polyphenol
content (expressed as mg of gallic acid equivalent per g of dry weight (mg GAE/g DW)), total
flavonoid content (expressed as mg of quercetin equivalent per g (mg QE/g DW)), total phenolic
acid content (expressed as mg caffeic acid content per g (mg CAE/g DW)).
It was found that total polyphenol compound content for S. asperum was from 26.84 to
105.14 mg GAE/g DW, for S. cauasicum from 65.59 to 113.58 mg GAE/g DW, for S. ×uplandicum
from 16.17 to 67.59 mg GAE/g depending on the stage of growth. Also, the total content of
flavonoids determined in extracts of S. asperum, S. cauasicum, and S. ×uplandicum was 13.23–
56.39, 23.05–52.86, and 2.71–19.76 mg QE/g DW, respectively. The total content of phenolic
acids was from 11.44 to 46.11 mg CAE/g DW for S. asperum, from 34.87 to 66.86 mg CAE/g DW
for S. cauasicum, and from 2.65 to 39.76 mg CAE/g DW for S. ×uplandicum depending on growth
period. In total, the content of each group of investigated compounds accumulated unevenly
during vegetation for all species. The peak of polyphenol content was determined at the start of
vegetation for S. asperum, at the stem growth for S. caucasicum, and at the budding for S.
×uplandicum.
Usually, the most pharmacological and biochemical studies of Symphytum genus
concerning S. officinale L. as world-widely know as a medicinal plant with healing features.
However, the results of this study also can be used in future pharmacological researches. Plant
raw of investigated species can be a rich source of polyphenol compounds along with other
species growing in natural and cultural conditions.

Keywords: Symphytum, polyphenols, flavonoid content, phenolic acid content.
Acknowledgments
This study was supported by the active participation of researches by the international network
AgroBioNet, Bilateral Scholarship of the Ministry of Education, Science, Research and Sport (Slovak
Republic), and Visegrad Fund.

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Spiritual Human Development
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