Page 144 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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LIGNIN AND SUGARS CONTENT IN THE RAW OF SELECTED POACEAE BARNHART
Olena Vergun, Dzhamal Rakhmetov, Valentyna Fishchenko, Svitlana Rakhmetova

M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine,
Kyiv, Ukraine; E-mail.: olenavergun8003@gmail.com

Last decades the search and study of new plants for energetic purposes has been
continued. In Ukraine, many-year investigations have been conducted in the M.M. Gryshko
National Botanical Garden (NBG) in the Department of Cultural Flora where collected
numerous species, varieties, and cultivars of perspective energetic plants. The complex study
focused on the biological, ecological, biochemical, and physiological properties of these plants.
The determination of the dry matter, the total content of sugars, ash content, caloricity, and
lignin content is an important characteristic in the investigation of the biochemical composition
of potent energetic plants.
Lignin is an aromatic heteropolymer predominantly composed of coniferyl and sinapyl
alcohol in angiosperms and contains additional p-coumaryl alcohol units in grasses. Lignins
from herbaceous plants have been studied not fully relatively with woody plants. Some
investigations showed that lignin in the Poaceae plants contains the methoxyl groups than
lignin from Fabaceae Lindl. or Caryophyllaceae Juss.
The study was conducted in the NBG. The content of dry matter, the total content of
sugars, and lignin were determined in raw of selected Poaceae plants: Eleusine coracana (L.)
Gaerth. Cv. Tropikanka, Hordeum bulbosum L., Miscanthus sacchariflorum (Maxim.) Benth.,
Panicum virgatum L., Setaria italica (L.) Beauv. spp. maxima (Alef.) Mansf., cv. s. Sviatkova, S.
italica Beauv. spp. mocharia (Alef.) Mansf. × S. italica (L.) Beauv. ssp. maxima (Alef.) Mansf., f.
ETSHCHMIF-3.1, Sorghum almum Parodi, S. bicolor (L.) Moench., S. × drummondii (Nees ex
Steud.), S. sudonense, S. saccharatum, cv. Pamat Shepelia. The investigation was carried out in
2015–2017 at the stage of flowering. The dry matter content was determined by drying plant
raw till constant weight at 105 ℃.. The reducing sugars content was found using Bertrand’s
method. The lignin content was detected by cellulose stabilization in sulfuric acid.
In the Department of Cultural Flora of the NBG have been studied plants with different
useful purposes, among which energetic plants. The collection fund of energetic plants includes
species, varieties, and cultivars of representatives of Miscanthus Anderss., Panicum L., Sorghum
Moench, Paulownia Siebold & Zucc., etc. The complex study of energetic plants found that the
content of dry matter for all investigated plants was from 20.40 (E. coracana) to 63.32 (H.
bulbosum) %. The content of reducing sugars was from 1.94 (S. italica, cv. s. Sviatkova) to 8.41
(S. bicolor) %. The lignin content was found in the range from 2.70 (S. × drummondii) to 14.37
(M. sacchariflorum) %. We found a very weak correlation between sugars and lignin of
investigation plants (r = 0.134). Al other parameters showed a negative correlation between
each other.
Results showed that at the flowering stage different Poaceae species accumulated high
content of dry matter, reducing sugars, and lignin. This study demonstrated the results of
primary biochemical investigations of selected plants from the Poaceae family that can be
useful for further biochemical study and selective work.

Keywords: Poaceae, lignin, sugars.

5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and |143
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Spiritual Human Development
November 3 2021
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