Page 138 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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TEST-INDICATORS OF LEPIDIUM SATIVUM L. SEEDLINGS UNDER THE INFLUENCE OF
MICELLAR WATER
Nataliia Tkachuk, Iryna Okulovich
T.H. Shevchenko National University "Chernihiv Colehium",
Chernihiv, Ukraine; E-mail.: nataliia.smykun@gmail.com
The use of plants to determine the toxicity of the environment (phytotesting) is widely
used in ecological research. In particular, the test-plant sensitive to toxicants is garden cress
(Lepidium sativum L.). Test-indicators of sensitive plants are the level of seed germination, the
value of mass, and the size of seedlings. Today is known, that surfactants are dangerous
pollutants. Thus, the synthetic surfactant is poloxamer 124, which together with other organic
compounds is part of cosmetics, in particular micellar water. Therefore, this work aimed to
study the biometric parameters of garden cress under the influence of cosmetic solutions of
micellar water.
The seeds of the test plant (L. sativum) 10 pieces were placed on filter paper moistened
with distilled water (control) or a suitable aqueous solution of micellar water (experiment). The
experiment was repeated three times. Investigated available in the retail network of Ukraine
means for removing makeup and cleansing the skin micellar water, which contained (according
to the manufacturer): aqua, PEG-40 hydrogenated castor oil, glycerin, Prunus (Amygdalus)
dulcis oil, panthenol, sorbitol, decyl glucoside, glyceryl glucoside, poloxamer 124, propylene
glycol, disodium cocoyl glutamate, sodium chloride, trisodium EDTA, polyquaternium-10, 1,2-
hexanediol, citric acid, sodium acetate, phenoxyethanol. The investigated concentrations of the
micellar water were 6.25 %, 12.5 %, 25.0 %, 50.0 % and 100 %. Seed germination energy (3rd
day), seed germination, and biometric-morphometric parameters (length of roots and
aboveground part of seedlings) (5th day) were determined. The phytotoxic effect and the
toxicity index of the solutions were calculated. The results were processed statistically using
Excel 2010, determining: arithmetic mean and arithmetic mean error; the significance of
differences according to Student’s t-test.
It was found that the germination rates of garden cress seeds and biometric indicators of
seedlings significantly decrease with the increasing concentration of the studied micellar water.
Thus, germination energy and seed germination decreased by 14–100 %. At the same time,
when watering the seeds of L. sativum with a solution with the maximum investigated
concentration (100 %), it did not germinate. The length of the aboveground part of garden cress
seedlings was determined only for the control and the variant with a concentration of micellar
water of 6.25 % (it was 2 times significantly less than in the control) because at other higher
concentrations the aboveground part of the seedlings was absent. There was a statistically
significant decrease in the length of the roots compared to the control: 1.2 times (6.25 %), 1.6
times (12.5 %), 2.6 times (25.0 %), 3.3 times (50 %). The phytotoxic effect ranged from 49.6 to
100 %. It is established that the value of the total toxicity index of solutions is from 0.55 (6.25
%) to 0 (100 %), indicating an increase in the toxicity of the solution with increasing
concentration.
Thus, garden cress L. sativum was a sensitive plant to the studied cosmetic. The obtained
data confirm the high efficiency of this test plant for use in biotesting. As this cosmetic is used
in small quantities and is significantly diluted with water when it enters the sewer system, its
toxic effects are likely to be small or non-existent.

Keywords: Lepidium sativum, micellar water, phytotesting.

5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and |137
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Spiritual Human Development
November 3 2021
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