Page 135 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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TOTAL ANTIOXIDANT CAPACITY IN THE MUSCLE TISSUE OF THE RAINBOW TROUT
(ONCORHYNCHUS MYKISS WALBAUM) AFTER IN VITRO INCUBATION WITH EXTRACTS
DERIVED FROM LEAVES OF VARIOUS CULTIVARS OF CAMELLIA JAPONICA L.

Halyna Tkachenko , Natalia Kurhaluk , Lyudmyla Buyun , Igor Kharchenko ,Maryna
1
2
1
2
Opryshko , Myroslava Maryniuk , Oleksandr Gyrenko
2
2
2
1 Institute of Biology and Earth Sciences, Pomeranian University in Słupsk, Poland;
E-mail.: halyna.tkachenko@apsl.edu.pl
2 M.M. Gryshko National Botanic Garden, National Academy of Science of Ukraine, Kyiv, Ukraine
In this study, extracts derived from leaves of various cultivars of Camellia japonica L.
plants were chosen because of their recorded potential medical significance with antimicrobial,
antioxidant, antitumor, anti-allergic, antiviral and skin healing properties. The main objective
of the present study was to evaluate the effect of extracts derived from the leaves of various C.
japonica cultivars on the total antioxidant capacity (TAC) level in the muscle tissue of rainbow
trout (Oncorhynchus mykiss Walbaum).
The extracts obtained from the leaves of various C. japonica cultivars (Kramer’s Supreme,
C.M.Wilson, La Pace, Mrs. Lyman Clarke, Benikarako, Fanny Bolis) from the living plant
collections grown under glasshouse conditions at M.M. Gryshko National Botanic Garden of the
National Academy of Sciences of Ukraine (Kyiv, Ukraine) were used. Freshly collected leaves
were washed, weighed, crushed, and homogenized in phosphate buffer 0.1М (in a ratio 1:19,
w/w) at ambient temperature. Muscle tissue was removed from rainbow trout after immediate
decapitation. The tissue samples were rinsed and homogenized in ice-cold 100 mМ Tris-HCl
buffer (pH 7.2). After centrifugation, the supernatant was used for incubation with leaf extracts
of various C. japonica cultivars (in ratio 19:1). The incubation time was 2 hrs., after that the TAC
was evaluated in the incubated homogenate.
Our results revealed quite a high TAC level in samples of muscle tissue incubated with leaf
extracts of C. japonica cultivars C.M. Wilson and Benikarako. The levels of ТAC were increased
by 41.7 and 44.8 % (p <0.05) as compared with the control samples. Leaf extracts of cultivars
La Pace and Kramer’s Supreme being incubated with muscle tissue have not changed the level
of ТAC, while the effect of the leaf extracts of cultivars Mrs. Lyman Clarke and Fanny Bolis on
the decrease of ТAC level was insignificant (p >0.05). The results of the study suggested the high
antioxidant capacity of Camellia cultivars screened give reason to believe that application of
these plant extracts signifies a rational curative strategy to prevent and cure various fish
diseases involving oxidative stress by increasing the ability of fish organism to adapt. It was
observed that the positive effect of Camellia’s preparations for the treatment of various
pathological conditions of the organism is associated with high content of the substances with
high antioxidant activity responsible for its ability to reduce oxidative stress. The obtained data
suggested, that the level of ТAC in samples of muscle tissue may be used as one of the indicators
in order to assess the functional conditions of antioxidant defenses of the organism and
effectiveness of exogenous antioxidant application. Thus, the obtained results, ascertained the
potency of the extracts from leaves of various C. japonica cultivars as a potential source of
natural antioxidant agents to be used in intensive aquaculture. Some discrepancy in scavenging
potential of the C. japonica leaf extracts screened in this study and bibliographic data reporting
profound biological activities of extracts derived from various part of this plant species may be
due to variation in the percentage of phytocompounds extracted in various solvents or to
different cultivation technology as well. To conclude, C. japonica may be used as an antioxidant
agent in aquaculture as it can be easily obtained and is not expensive. Furthermore, the use of
such plants products in aquaculture systems may also have environmental value because of
their biodegradability.

Keywords: Camellia, leaves, extracts, total antioxidant capacity, rainbow trout.

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5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and |134
Spiritual Human Development
November 3 2021
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