Page 134 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

th

ANTI-HEMOLYTIC EFFECTS OF EXTRACTS OBTAINED FROM LEAVES OF VARIOUS
BEGONIA L. SPECIES ON THE EQUINE ERYTHROCYTE MEMBRANES
2
2
Halyna Tkachenko , Lyudmyla Buyun , Natalia Kurhaluk , Maryna Opryshko ,
1
1
Myroslava Maryniuk , Oleksandr Gyrenko
2
2
1 Institute of Biology and Earth Sciences, Pomeranian University in Słupsk, Poland;
E-mail.: halyna.tkachenko@apsl.edu.pl
2 M.M. Gryshko National Botanic Garden, National Academy of Science of Ukraine, Kyiv, Ukraine
In the present study, the percentage hemolysis of equine erythrocytes induced by
treatment with leaf extracts of various Begonia species are determined to exemplify their
further potential development and use as alternative plant-derived agents against metabolic
diseases in medicine and veterinary.
The leaves of Begonia plants, cultivated under glasshouse conditions, were sampled at
M.M. Gryshko National Botanical Garden, National Academy of Science of Ukraine. The leaves
of thirty plants species, i.e. Begonia foliosa Kunth, B. psilophylla Irmsch., B. convolvulacea
(Klotzsch) A.DC., B. ulmifolia Willd., B. rex Putz., B. thiemei C.DC., B. manicata Brongn., B.
solimutata L.B.Sm. & Wassh., B. arborescens var. oxyphylla (A.DC.) S.F.Sm., B. mexicana G. Karst.
ex Fotsch, Begonia × credneri F.Haage & E.Schmidt, B. sanguinea Raddi, B. olbia Kerch., B.
goegoensis N.E.Br., B. imperialis var. smaragdina Lem., B. epipsila Brade, B. pustulata Liebm., B.
heracleifolia var. nigricans Hook.f., Begonia × erythrophylla Hérincq, B. aconitifolia A.DC., B.
peltata Otto & Dietr., B. nelumbiifolia Cham. & Schltdl., B. subvillosa Klotzsch, B. oxyphylla A.DC.,
B. masoniana Irmsch. ex Ziesenh, B. cucullata Willd., B. angularis Raddi, B. glabra Aubl., B.
boisiana Gagnep., B. venosa Skan ex Hook.f. were sampled for study. Freshly collected leaves
were washed, weighed, crushed, and homogenized in 0.1M phosphate buffer (pH 7.4) (in ratio
1:19, w/w) at room temperature. To evaluate the potential of extracts to cause hemolysis in
equine erythrocytes, a hemolysis assay based on the spectrophotometric measurement of
hemoglobin in the supernatant was performed. The extent of hemolysis was determined by
measurement of the absorbance at 540 nm corresponding to hemoglobin liberation.
Our study demonstrated that among 30 species of the Begonia genus, the most species of
plants investigated possessed anti-hemolytic activity. The results of these biological assays
demonstrated that compounds present in B. glabra, B. aconitifolia, B. sanguinea, B. thiemei, B.
masoniana, B. × credneri, B. oxyphylla, B. subvillosa, B. ulmifolia, B. conconvulaceae can cause the
prevention of formation of methemoglobin and reduce of hemolysis, while B. erythrophylla, B.
psilophylla and B. arborescens var. oxyphylla extracts can induce the formation of
methemoglobin and cause hemolysis in healthy equine blood. Exposure to extracts from leaves
of B. foliosa, B. rex, B. solimutata, B. mexicana, B. goegoensis, B. imperialis var. smaragdina, B.
pustulata, B. peltata, B. cucullata, B. angularis, B. boisiana, B. venosa exhibited the decrease of
percentage hemolysis of equine erythrocytes, but these alterations were non-significant.
The extensive use of plants from this genus having ethnobotanical value by the local
people in treating various types of diseases and disorders might be justified by their antioxidant
activities against oxidative stress and hemolysis, which are known to be responsible for causing
various metabolic states and diseases. Further studies aimed at the isolation and identification
of active substances from the various species of the Begonia genus, as well as assessment of
oxidative stress biomarkers could also disclose compounds with better therapeutic value and
doses.

Keywords: Begonia, leaves, extracts, hemolysis, equine erythrocytes.
Acknowledgments
We are grateful to The Polish National Commission for UNESCO for supporting our study.

5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and |133
th
Spiritual Human Development
November 3 2021
rd