Page 137 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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COMPARATIVE ASSESSMENT OF ANTIMICROBIAL EFFICACY OF ETHANOLIC EXTRACTS
FROM LEAVES OF CAMELLIA JAPONICA L. CULTIVARS AGAINST STAPHYLOCOCCUS
AUREUS STRAIN

Halyna Tkachenko , Natalia Kurhaluk , Igor Kharchenko , Lyudmyla Buyun , Maryna
1
1
2
2
Opryshko , Myroslava Maryniuk , Oleksandr Gyrenko
2
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2
1 Institute of Biology and Earth Sciences, Pomeranian University in Słupsk, Poland;
E-mail.: halyna.tkachenko@apsl.edu.pl
2 M.M. Gryshko National Botanic Garden, National Academy of Science of Ukraine, Kyiv, Ukraine
According to the estimation of Erasmus+ project Good Herbs (http://good-herbs.eu/),
about 90 % of species are harvested from wild flora and only 10 % are cultivated commercially.
This work is a continuation of a series of publications directed towards in vitro assessment of
the antibacterial potentials of Camellia L. plants from living plant collections maintained at M.M.
Gryshko National Botanical Garden (NBG). Therefore, in this study, we aimed to determine the
antibacterial activity of six plant cultivars of Camellia japonica L., i.e. Kramer’s Supreme, C.M.
Wilson, La Pace, Mrs. Lyman Clarke, Benikarako, Fanny Bolis against Staphylococcus aureus
subsp. aureus Rosenbach (ATCC 29213™) strain.
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The leaves of C. japonica and its cultivars Kramer’s Supreme, C.M. Wilson, La Pace, Mrs.
Lyman Clarke, Benikarako, Fanny Bolis plants cultivated at NBG's glasshouses under natural
light, were sampled. The C. japonica cultivars included in this study represent four various
double flowers types, i.e. "paeony" ('Kramer’s Supreme' and ‘Benikarako’), "rose" ('C.M. Wilson'
and 'La Pace'), "semi-double" ('Mrs. Lyman Clarke'), and "formal double" (‘Fanny Bolis’).
Freshly collected leaves were washed, crushed, weighed, and homogenized in 96 % ethanol (in
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proportion 1:19) at room temperature. The S. aureus subsp. aureus Rosenbach (ATCC 29213™)
strain was used in the current study. The antimicrobial susceptibility testing was done by the
disc diffusion method (Kirby-Bauer disk diffusion susceptibility test protocol). After incubation
in an appropriate culture medium (Muller-Hinton agar), the diameter of the inhibition zone was
measured and averaged to assess the antibacterial efficacy.
The crude extracts were analyzed for their antibacterial effect by assessing their
inhibitory zones against S. aureus subsp. aureus strain. Among the six plant extracts screened,
C. japonica 'Mrs. Lyman Clarke' and ‘Benikarako’ exhibited the highest inhibitory zones against
the tested strain (the mean of the zone of inhibitions was 14.87 ±1.0 mm and 14.56 ±1.12 mm,
respectively). The intermediate activity was presented by variety C. japonica (13.78 ±1.12 mm),
‘Fanny Bolis’ (13.74 ±0.85 mm), 'Kramer’s Supreme' (13.1 ±0.98 mm), 'La Pace' (12.56 ±1.08
mm), and 'C.M. Wilson' (10.25 ±0.4 mm). The antibacterial effect of positive control was also
recorded (the mean value of the inhibition zone was 8.56 ±0.75 mm). The results obtained in
the current study are in line with early reports. The potential presence of naturally occurring
antimicrobials in petals of C. japonica active against foodborne pathogens in microbiological
media and food was studied by Kim et al. (2001).
In conclusion, the alcoholic leaf extracts of C. japonica and its cultivars revealed mild
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antibacterial activity against S. aureus subsp. aureus Rosenbach (ATCC 29213™) strain. The
antimicrobial ability of various samples of these plants might be due to a wide variety of
compounds. The findings reported herein give scientific credence to the traditional use of these
plants and suggest that extracts derived from the leaves of C. japonica and its cultivars merit
further chemical study as natural antibiotics to identify the secondary metabolites.

Keywords: Camellia japonica, leaves, ethanolic extract, antimicrobial activity, disk diffusion assay.
Acknowledgments
We are grateful to The Polish National Commission for UNESCO for supporting our study.

5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and |136
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Spiritual Human Development
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