Page 136 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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CHANGES IN OXIDATIVE STRESS BIOMARKERS IN THE EQUINE BLOOD TREATED IN
VITRO BY EXTRACT DERIVED FROM SANSEVIERIA PARVA N.E.BR.
Halyna Tkachenko , Natalia Kurhaluk , Lyudmyla Buyun ,Maryna Opryshko ,
1
2
1
2
Myroslava Maryniuk , Oleksandr Gyrenko
2
2
1 Institute of Biology and Earth Sciences, Pomeranian University in Słupsk, Poland;
E-mail.: halyna.tkachenko@apsl.edu.pl
2 M.M. Gryshko National Botanic Garden, National Academy of Science of Ukraine, Kyiv, Ukraine
In this study, we have focused on the antioxidant effect of leaf extract obtained from
Sansevieria parva N.E.Br. on oxidative stress biomarkers (2-thiobarbituric acid reactive
substances (TBARS), carbonyl derivatives content of protein oxidative modification) and
antioxidant defenses (total antioxidant capacity) using the equine erythrocytes as an
experimental model. Thus, equine erythrocytes were proved to be a good tool for analyzing the
oxidative stress biomarkers as predictors of antioxidant action of S. parva leaf extract.
The leaves of S. parva plant, cultivated under glasshouse conditions, were sampled at M.M.
Gryshko National Botanical Garden (NBG), National Academy of Science of Ukraine. Freshly
collected leaves were washed, weighed, crushed, and homogenized in 0.1M phosphate buffer
(pH 7.4) (in ratio 1:19, w/w) at room temperature. The pellet of blood was resuspended in 4
mM phosphate buffer (pH 7.4). A volume of 0.1 ml of the plant extract was added to 1.9 ml of
clean equine erythrocytes. For positive control, phosphate buffer was used. The level of lipid
peroxidation was determined by quantifying the concentration of 2-thiobarbituric acid reacting
substances (TBARS) with the Kamyshnikov (2004) method. The rate of protein oxidative
destruction was estimated from the reaction of the resultant carbonyl derivatives of amino acid
reaction with 2,4-dinitrophenylhydrazine as described by Levine et al. (1990) with
modification of Dubinina et al. (1995). Total antioxidant capacity (TAC) in the samples was
estimated by measuring the TBARS level after the Tween 80 oxidation (Galaktionova et al.,
1998). All statistical calculation was performed on separate data with STATISTICA 8.0 software
(StatSoft, Krakow, Poland).
The addition of the S. parva leaf extract for incubation with erythrocyte suspension caused
a non-considerable increase in TBARS formation (by 17 %, p >0.05), while the content of
aldehydic and ketonic derivatives of oxidatively modified proteins was decreased (by 0.7 %, p
>0.05 and 2.8 %, p <0.05, respectively) compared to control samples. The TAC level increased
by 11.6 % (p >0.05).
It is well evidenced that Sansevieria species have a wide range of secondary metabolites
and are traditionally used in the treatment of various diseases and disorders. Recently, the
investigations of Thu et al. (2020) have been succeeded by the isolation and identification of
hundreds of phytochemical constituents isolated from Dracaena and Sansevieria. The most
characteristic metabolites are steroids, flavonoids, stilbenes, and saponins; many of them
exhibit potent analgesic, anti-inflammatory, antimicrobial, antioxidant, antiproliferative, and
cytotoxic activities. Thus, the results of the present study reinforce the importance of the
analyzed plants as a source of bioactive compounds with antioxidant properties. Further
chemical analysis of the aforementioned plant extract should be performed to determine their
chemical composition and identify the exact phytocompounds responsible for antioxidant
activity. In addition, they should be subjected to pharmacological evaluations to assess theirs
in vivo efficacy, toxicity, potential adverse effects, interactions, and contraindications.

Keywords: Sansevieria parva, leaves, extracts, oxidative stress, equine erythrocytes.
Acknowledgments
We are grateful to The Polish National Commission for UNESCO for supporting our study.

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5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and |135
Spiritual Human Development
November 3 2021
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