Page 129 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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IN VITRO EVALUATION OF ANTIMICROBIAL EFFICACY OF ETHANOLIC EXTRACTS FROM
LEAVES OF FICUS TINCTORIA G. FORST.
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2
1
Halyna Tkachenko , Lyudmyla Buyun , Natalia Kurhaluk , Anna Góralczyk , Vitaliy
1
Honcharenko , Andriy Prokopiv
3
3,4
1 Institute of Biology and Earth Sciences, Pomeranian University in Słupsk, Poland;
E-mail.: halyna.tkachenko@apsl.edu.pl
2 M.M. Gryshko National Botanic Garden, National Academy of Science of Ukraine, Kyiv, Ukraine
3 Ivan Franko National University of Lviv, Ukraine
4 Botanic Garden of Ivan Franko National University of Lviv, Ukraine
The current study is a continuous line of our investigations directed towards the
assessment of the antibacterial potentials of Ficus species plants. The current study aimed to
determine the antibacterial activity of Ficus tinctoria G. Forst. against Gram-positive and Gram-
negative bacteria. For this study, а panel of organisms including Staphylococcus aureus subsp.
aureus Rosenbach (ATCC 25923™) (mecA negative), S. aureus subsp. aureus Rosenbach
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(ATCC 29213™) (mecA negative, Oxacillin sensitive, weak β-lactamase producing strain), S.
aureus NCTC 12493 (mecA positive, Methicillin-resistant, EUCAST QC strain for cefoxitin),
Escherichia coli (Migula) Castellani and Chalmers (ATCC 25922™), E. coli (Migula) Castellani
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and Chalmers (ATCC 35218™), Pseudomonas aeruginosa (Schroeter) Migula (ATCC 27583™)
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were used. The antimicrobial susceptibility testing was done on Muller-Hinton agar by the disc
diffusion method. Inhibition zone diameters were determined and averaged.
It was observed, that the ethanolic extract obtained from leaves of F. tinctoria resulted in
considerable growth suppression of S. aureus and E. coli strains. Moreover, the differential
efficacy of ethanolic extract derived from leaves of F. tinctoria plant on the test organisms was
evidenced. Consequently, the extract screened displayed high antibacterial potency against S.
aureus, i.e. the mean of inhibition zone diameters was (13.49 ±1.08) and (17.74 ±1.13) mm for
S. aureus subsp. aureus (ATCC 29213™) and S. aureus NCTC 12493, respectively. Also, E. coli
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exhibited similar susceptibility for the impact of the ethanolic extract obtained from leaves of
F. tinctoria. In particular, the mean values of inhibition zone diameters were (16.25 ±1.12) and
(15.34 ±0.91) mm for E. coli (ATCC 25922™) and E. coli (ATCC 35218™), respectively. P.
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aeruginosa (ATCC 27583™) strain was the most resistant to the impact of the ethanolic extract
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obtained from leaves of F. tinctoria with the mean of inhibition zone diameter (10.33 ±0.65)
mm. Moreover, the ethanolic extract obtained from leaves of F. tinctoria revealed significant
antibacterial activity against studied strains compared to control samples (96 % ethanol). A
statistically significant increase (p<0.05) in inhibition zone diameters of strain growth was 58
% (for S. aureus subsp. aureus ATCC 29213™) and 94.5 % (for S. aureus NCTC 12493). A
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significant increase (p<0.05) in inhibition zone diameters for growth of E.coli strains was also
observed (by 84 % for E. coli ATCC 25922™ and by 71 % for E. coli ATCC 35218™,
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respectively).
Thus, the present study has revealed that ethanolic extract derived from the leaves of F.
tinctoria exhibited significant antibacterial activity against different both Gram-positive and
Gram-negative strains studied (inhibition zone diameter ranged from 8.3 to 19.7 mm). The
promising results on medicinal plants screening for antibacterial activity could be considered
as primary information for further phytochemical and pharmacological studies. In particular,
the next step in our further investigation will be HPLC-profiling of the plant extract to find new
bioactive compounds from a natural source.
Keywords: Ficus tinctoria, leaves, antibacterial efficacy, disc diffusion technique, ethanolic extracts.

Acknowledgments
We are grateful to The Visegrad Fund for supporting our study.

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5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and |128
Spiritual Human Development
November 3 2021
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