Page 132 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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IN VITRO ESTIMATION OF TOTAL ANTIOXIDANT CAPACITY OF THE MUSCLE TISSUE OF
RAINBOW TROUT (ONCORHYNCHUS MYKISS WALBAUM) EXPOSED TO LEAF EXTRACTS
OF SANSEVIERIA SUFFRUTICOSA N.E.BR.

Halyna Tkachenko , Lyudmyla Buyun , Natalia Kurhaluk , Maryna Opryshko ,
2
1
2
1
Myroslava Maryniuk , Oleksandr Gyrenko
2
2
1 Institute of Biology and Earth Sciences, Pomeranian University in Słupsk, Poland;
E-mail.: halyna.tkachenko@apsl.edu.pl
2 M.M. Gryshko National Botanic Garden, National Academy of Science of Ukraine, Kyiv, Ukraine
It is believed that the total antioxidant capacity (TAC) can be considered as a marker of
oxidative stress, which can be used in biomedical and nutritional studies, since it measures the
state of antioxidant capacity in biological tissues. The aim of this study was to evaluate an in
vitro effect of extract obtained from leaves of Sansevieria suffruticosa N.E.Br. on the total
antioxidant capacity (TAC) in the muscle tissue of the rainbow trout (Oncorhynchus mykiss
Walbaum).
The leaves of S. suffruticosa plants, cultivated under glasshouse conditions, were sampled
at M.M. Gryshko National Botanical Garden, NAS of Ukraine. Freshly collected leaves were
washed, weighed, crushed, and homogenized in 0.1M phosphate buffer (pH 7.4). The minced
muscle tissue of clinically healthy rainbow trout was used to incubate with extract of S.
suffruticosa (in a ratio 19:1, v/v) at room temperature. The incubation time was 2 hours. TAC
was assessed in the incubated homogenate by measuring the 2- thiobarbituric acid reactive
substances (TBARS) level after Tween-80 oxidation. according to Galaktionova et al (1998).
Significance of differences between the oxidative stress biomarkers level (significance level,
p<0.05) was examined using the Mann-Whitney U test (StatSoft, Poland).
The results showed that extract of S. suffruticosa leaf efficiently increased the TAC level in
muscle tissue by 66.8 % (p<0.05), suggesting that the extract of S. suffruticosa has possessed
remarkable antioxidant potential. According to the abovementioned antioxidant mechanisms,
extract of S. suffruticosa may activate antioxidant enzymes and their synthesis de novo.
According to the results obtained, we addressed the hypothesis that by-products in the extract
of various S. suffruticosa may be a major contributor to the increase of antioxidant capacity of
muscle tissue of rainbow trout after incubation in vitro. To prove this hypothesis, separation
and characterization of secondary compounds in plant extracts are required for further study.
In fact, different antioxidant effects of various species of the Sansevieria genus are determined
by their by-products (alkaloids, flavonoids, saponins, glycosides, terpenoids, tannins, proteins,
carbohydrates, etc.). Indeed, the study on S. roxburghiana and S. trifasciata has revealed the
presence of important compounds which were separated by thin-layer chromatography.
Phytochemical screening of the extracts of the S. trifasciata plant showed the presence of major
classes of phytochemicals.
Taking into account existing experimental evidence, it is reasonable to assume that
secondary plant metabolites, i.e. polyphenolic compounds in extract of S. suffruticosa may
contribute to the antioxidant activity. In conclusion, the results of this study provide a new
perspective for the use of various Sansevieria species as a medicinal plant to improve the
antioxidant response of rainbow trout. Further studies including the use of other medicinal
plants as food additives in aquaculture, the assessment of its antioxidant effects on various
tissues are in progress.

Keywords: Sansevieria suffruticosa, leaves, extracts, total antioxidant capacity, rainbow trout.
Acknowledgments
We are grateful to The Polish National Commission for UNESCO for supporting our study.

5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and |131
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Spiritual Human Development
November 3 2021
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