Page 131 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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ANTIBACTERIAL ACTIVITY OF SANSEVIERIA AETHIOPICA THUNB. LEAF EXTRACT
Halyna Tkachenko , Lyudmyla Buyun , Natalia Kurhaluk , Myroslava Maryniuk
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1 Institute of Biology and Earth Sciences, Pomeranian University in Słupsk, Poland;
E-mail.: halyna.tkachenko@apsl.edu.pl
2 M.M. Gryshko National Botanic Garden, National Academy of Science of Ukraine, Kyiv, Ukraine
Several African medicinal plants previously investigated for biological potential showed
good antibacterial activities. Some of them include species belonging to the Sansevieria genus.
In our continuous search of phytochemicals to combat bacterial infections, we designed the
current study to evaluate the antimicrobial potential of Sansevieria aethiopica against Gram-
negative and Gram-positive phenotypes, i.e. Escherichia coli, Staphylococcus aureus, and
Pseudomonas aeruginosa strains, clinically important bacteria, which are indicator organisms
commonly used in various projects in order to monitor antibiotic resistance.
Freshly collected leaves were washed, weighed, crushed, and homogenized in 96 %
ethanol (in proportion 1:19) at room temperature. The extracts were then filtered and
investigated for their antimicrobial activity. All extracts were stored at 4°C until use. For this
study, а panel of organisms including Staphylococcus aureus subsp. aureus Rosenbach
(ATCC 25923™) (mecA negative), S. aureus subsp. aureus Rosenbach (ATCC 29213™) (mecA
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negative, Oxacillin sensitive, weak β-lactamase producing strain), S. aureus NCTC 12493 (mecA
positive, Methicillin-resistant, EUCAST QC strain for cefoxitin), Escherichia coli (Migula)
Castellani and Chalmers (ATCC 25922™), E. coli (Migula) Castellani and Chalmers
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(ATCC 35218™), Pseudomonas aeruginosa (Schroeter) Migula (ATCC 27583™) was used.
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Antimicrobial activity was determined using the agar disk diffusion assay. The following
inhibition zone diameter criteria were used to assign susceptibility or resistance of bacteria to
the phytochemicals tested: Susceptible (S) ≥ 15 mm, Intermediate (I) = 10–15 mm, and
Resistant (R) ≤ 10 mm.
In line with the growing interest in the antibacterial potential of different plants, we
examined the antibacterial properties of S. aethiopica leaves against E. coli, S. aureus, and P.
aeruginosa strains. The results of antibacterial activity screening indicate that the extract has
shown antibacterial activity against all tested organisms. The leaf extract has shown better
activity against S. aureus strains compared to the E. coli and P. aeruginosa strains. The diameters
of inhibition zones were (26.35 ±1.26) mm, (16.15 ±1.47) mm, and (21.6 ±1.23) mm for S.
aureus ATCC 25923™, S. aureus ATCC 29213™, and S. aureus NCTC 12493, respectively. The
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extract has shown less antimicrobial activity against P. aeruginosa. The mean value of the
inhibition zone was (12.49 ±1.09) mm. Finally, the ethanolic extract exhibited mild antibacterial
activity against E. coli (mean value of inhibition zone ranged within (18.62 ±1.32) mm for E. coli
ATCC 25922™ and (16.38 ±1.02) mm for E. coli ATCC 35218™).
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Clear inhibition zones indicated that the compounds showed the antibacterial activity of
the antibiotic disc against bacterial strains. It was observed that strains of both Gram-positive
and Gram-negative strains: E. coli, P. aeruginosa, and S. aureus were sensitive to S. aethiopica
extract. Therefore, it is concluded that plant extract possesses antibacterial activity against
tested organisms. The values of the inhibition zone varied suggesting the various degree of
efficacy and different substances of the extract towards the target strains. Additionally, the
antibacterial activity of the S. aethiopica extract may be due to the presence of various active
metabolites. Thus, the preliminary antibacterial screening indicated that the leaves of S.
aethiopica with antibacterial properties may offer alternative therapeutic agents against
bacterial infections.
Keywords: Sansevieria aethiopica, leaves, ethanolic extract, antimicrobial activity, disk diffusion
method.

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Spiritual Human Development
November 3 2021
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