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5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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ANTIOXIDANT ACTIVITY OF ETHANOL EXTRACTS OF SELECTED ASTERACEAE BERCHT.
& J. PRESL REPRESENTATIVES
1
Olena Vergun , Oksana Shymanska , Evá Ivanišová , Ján Brindza
2
1
2
1 M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine, Kyiv,
Ukraine; E-mail.: olenavergun8003@gmail.com
2 University of Life Sciences, Lublin, Poland
Asteraceae Bercht. & J. Presl is a very large family, representatives of which known as
medicinal, forage, ornamental, energetic, etc., plants. Numerous studies of different Asteraceae
species demonstrated significant pharmaceutical and medicinal potential. The biological
activities of extracts of these plants are caused by the presence of various compounds such as
polysaccharides, terpenes, fatty acids, phytosterols, lignans, saponins, etc. Plant extracts
exhibited antimicrobial, anti-inflammatory, antioxidant, hepatoprotective, cardioprotective
activities.
This study aimed to determine the content of groups of polyphenol compounds and
antioxidant activity of Achillea nobilis L., Echinops ritro L., E. sphaerocephalus L., Inula helenium
L., Tanacetum vulgare L., Taraxacum campylodes G.E. Haglund extracts at the flowering. In the
experiment used plant raw collected from the natural flora of M.M. Gryshko National Botanical
Garden of the NAS of Ukraine during 2019–2020 at the flowering stage. All investigations were
conducted in the Slovak University of Agriculture in Nitra (Slovak Republic). Ethanol extracts of
investigated plants were measured for total polyphenol content (expressed as mg of gallic acid
equivalent per g of dry weight (mg GAE/g DW)), total flavonoid content (expressed as mg of
quercetin equivalent per g (mg QE/g DW)), total phenolic acid content (expressed as mg caffeic
acid content per g (mg CAE/g DW)). Antioxidant activity measured by DPPH and
phosphomolybdenum methods (expressed as mg Trolox equivalent per g (mg TE/g DW)).
These species were selected due to their significant therapeutical properties, especially in
folk medicine. It was determined total phenolic content from 34.84 (A. nobilis) to 74.39 (T.
vulgare) mg GAE/g DW, total flavonoid content from 23.70 (A. nobilis) to 41.43 (T. vulgare) mg
QE/g DW, total phenolic acid content from 10.13 (A. nobilis) to 28.94 (T. vulgare) mg CAE/g DW.
Antioxidant activity by DPPH method was from 5.28 (I. helenium) to 6.84 (E. ritro) mg TE/g DW
and phosphomolybdenum method from 64.15 (A. nobilis) to 153.28 (E. sphaerocephalus) mg
TE/g DW.
All investigated plant species are a rich source of polyphenol compounds and
characterized by high antioxidant activity. Obtained data can be useful for further biochemical
and pharmacological investigations of Asteraceae representatives. Plants from natural flora can
be a potential source of antioxidants.

Keywords: Asteraceae, polyphenols, antioxidant activity.
Acknowledgments
This study was supported by the active participation of researches by the international network
AgroBioNet, Bilateral Scholarship of the Ministry of Education, Science, Research and Sport (Slovak
Republic), and Visegrad Fund.

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Spiritual Human Development
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