Page 145 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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BIOCHEMICAL CHARACTERISTIC OF CICER ARIETINUM L. GENOTYPES
Olena Vergun, Dzhamal Rakhmetov, Svitlana Rakhmetova, Oleksandr Bondarchuk,
Valentyna Fishchenko
M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine
Kyiv, Ukraine; E-mail.: olenavergun8003@gmail.com
Cicer arietinum L. (chickpea) is an ancient crop that is cultivated widely in tropical,
subtropical regions. This legume is one of the most important food plants in the world with
high-quality protein, especially for vegetarian people. In Asia global production of chickpea is
approximately 60 %. The biochemical composition of C. arietinum is fatty acids, minerals, β-
carotenes, and bioactive compounds are flavonoids, phenolic acids, lignin, carotenoids. Selected
components of C. arietinum have a potential beneficial effect in decrease the risk of some
chronic diseases.
This study was aimed to determine selected biochemical parameters in the raw of C.
arietinum genotypes: f. CAOCHL, f. CATADJD-2, f. CAUKR, f. CAAZEUR-2, f. CAAFGD-2, f.
CAAZEMR-1, f. CABFR-2, f. CABFR-1, f. CAAFGK-1 at the end of flowering–start of fruitage (EFFr)
and fruitage–start of ripening (FrSR) during 2020–2021. Plant raw material was collected from
the experimental collections of M.M. Gryshko National Botanical Garden of the NAS of Ukraine.
The content of dry matter is determined after drying at 105 °C till constant weight. The reducing
sugar content was detected by Bertrand’s method. Ascorbic acid content determined in acid
extracts by Tillman’s method. Titrable acidity and tannin content were determined from water
extracts by titration with an alkaline solution and indigo carmine solution, respectively. The
content of chlorophyll a, chlorophyll b, and carotenoids was determined after measuring the
optic density of acetone solutions on spectrophotometer Unico and results were given as mg/g
of fresh weight. Results showed that dry matter in raw of 6 genotypes decreased from EFFr to
FrSR. In total, the content of dry matter was 26.62–35.56 % at EFFr and 22.10–33.74 % at FrSR.
Reducing sugars content was 7.39–12.81 % at EFFr and 6.47–10.96 % at FrSR. The ascorbic acid
content for investigated genotypes was from 35.33 to 217.94 mg% at EFFr and from 52.13 to
169.80 mg% at FrSR. The titrable acidity was from 2.32 to 3.19 % at EFFr and from 1.41 to 3.93
% at FrSR. Content of tannins was from 1.04 to 1.55 % at EFFr and from 0.21 to 6.70 % at FrSR.
As a result of the study, the content of chlorophyll a was from 1.11 to 2.61 mg/g at the end of
blossoming-fruiting and from 0.82 to 2.04 mg/g at the fruiting-ripening stage. The chlorophyll
a content was significantly higher than chlorophyll b. The content of chlorophyll b was from
0.297 to 0.426 mg/g and from 0.09 to 0.24 mg/g at the fruiting-ripening period. Carotenoids
accumulated at the end of blossoming-fruiting from 0.56 to 1.28 mg/g and the fruiting-ripening
stage from 0.47 to 1.29 mg/g. The relationship of chlorophylls content (a/b) was 3.28–7.48 at
the end of blossoming-fruiting and 4.28–15.24 at the fruiting-ripening stage. This ratio shows
the light adaptation response. The relationship between chlorophylls and carotenoids was
2.05–2.63 at the end of blossoming-fruiting and 1.67–2.59 at the fruiting-ripening stage. This
ratio is an indicator of the greenness of the plant and less values say about the senescence
process.
Thus, our study demonstrated that different genotypes of C. arietinum are a good source
of nutrients. Despite on wide use of this species in the food industry of Asian countries, the study
of biochemical composition and physiological parameters in other areas in the world. This study
can be useful for further selective work with this species and deep biochemical investigation.

Keywords: Cicer arietinum, biochemical composition, chlorophylls, carotenoids.

5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and |144
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Spiritual Human Development
November 3 2021
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