Page 151 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

th

INFLUENCE OF TALAROMYCES RUBER ON SEED GERMINATION OF
ZEA MAYS L.
Natalia Zaimenko, Nadiia Rositska, Bogdana Ivanytska
M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine,
Kyiv, Ukraine; E-mail.: rositska.nadiia@gmail.com
Phytopathogens are reported to be present in all the cereal growing regions of the world,
and not only affect the growth and seedling survival, but also reduce the quality and production
of cereals. Nearly 70–80 % of crop diseases by fungal phytopathogens causing significant
reduction in yield and quality of cereal crops, fruits and vegetables. Association of fungal
phytopathogens with cereals due to secretion of variety of metabolites and toxins means
harmful influence to humans and animals posing threat to agricultural products as become unfit
for human consumption. Most studies showed the major effect on seeds include reduced
germination, discoloration, visible mould growth, musty or sour odour, dry matter loss and
caking and mycotoxins elaboration. Contaminated agricultural products particularly cereals
are the main source of mycotoxins in the animal and human food chain. The genera of
Talaromyces commonly associated with crop plants are known to produce diverse toxic
substances causing different diseases in plants and animals including humans. The inhibitory
effect of the seed-borne fungi on seed germination, radicle and coleoptile growth has been
attributed to the production of certain enzymes and toxins produced by fungi in different crops.
The toxic effect of Talaromyces ruber was determined on corn seeds. T. ruber was grown
in 250 ml of Erlenmayer conical flask containing 100ml CYA broth for 12 days on rotary shaker
(LM-450D) at 27 ±2 °C. At the end of incubation period, culture filtrates were filtered through
Whatman filter paper no. 1 and centrifuged at 12,000 g to get cell-free filtrates. Hundred healthy
surface sterilized seeds were suspended in 50 ml of culture filtrates and incubated at 27 ±2 °C
for 24 hours and transferred to sterile-petri plates containing three layered wet blotter paper
and incubated for 5 days under illumination. Seeds soaked in uninoculated broth were served
as control. At the end of the incubation period, seed germination and radicle length were
measured. Each experiment was run in triplicate (n=3) and the results are statistically analysed
and expressed mean and standard deviation.
The present attempts to screen T. ruber in its toxic effect on corn seed germination
showed significantly retard the seed germination, radicle elongation. Toxic fungal metabolites
also induced adverse effects on plants such as inhibition of seed germination, malformation and
reducing seedlings. Many fungal toxins are known to be phytotoxic and play role in plant
pathogenesis. Very little information is available on the phytotoxic effects of Talaromyces
species. In the present investigations, we recorded that the culture filtrate of T. ruber was highly
toxic and caused a significant percentage of inhibition of the seed germination. The mean
inhibition of germination was 42.86 %. The mean inhibition of radicle growth was 46.67 %.
From the present investigations, it can be concluded that T. ruber was toxic and inhibited
the seed germination of Z. mays. In present observations, T. ruber also showed significant effects
on radicle growth of corn seeds.

Keywords: Talaromyces ruber, Zea mays, germination.

th
5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and |150
Spiritual Human Development
November 3 2021
rd