Page 83 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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VIABILITY OF BEES UNDER CONSUMPTION OF DIFFERENT DOSES OF MAGNESIUM
CITRATE
1
Iryna Kovalchuk , Maria Tsap , Ryslan Androshulik , Anastasia Kroh
2
1
2
1 Lvivskyy National University of Veterinary Medicine and Biotechnology named S.Z.Hzhytskoho, Lviv, Ukraine;
E-mail.: irenakovalchuk@ukr.net
2 Institute of Animal Biology National Academy of Agrarian Sciences of Ukraine, Lviv, Ukraine
It is known that the resistance of honey bees and the reproductive function of queen bees
largely depend on both genetic and biological and nutritional factors, including the level of
mineral nutrition. It is known that Mg and other mineral elements maintain the osmotic
pressure in the cell, provide transport of inorganic ions through their membranes, affecting
metabolic processes at the level of tissues, organs, and systems, which determines the
resistance of the organism. Therefore, the study of separate and combined use of mineral
elements, the introduction of a sugar syrup such as Mg citrate in feeding bees, their impact
resistance of the organism are relevant.
The research was conducted on four groups of bees 25–30 each. Bees isolated in the
control group (I) were fed 1 ml of 50 % sugar syrup (CA) and 1 ml of H2O daily; Group II
(experimental) – 1 ml of sugar syrup with the addition of 1 ml of Mg citrate containing 0.04 mg
Mg / l; Group III (experimental) – similarly with the addition of 1 ml of Mg citrate (0.02 mg Mg
/ l); Group IV (experimental) – similarly with the addition of 1 ml of Mg citrate (0.01 mg Mg /
l). The bees of the control and experimental groups were kept in similar conditions of the
laboratory thermostat TS-80M-3 with micro ventilation at a temperature of 30.00C for 30 days
of research. During the study period, daily control of the number of live and dead bees, their
motor and forage activity was performed. On the 30th day, journal records were compared with
the actual number of live and dead bees and the daily dynamics of survival and life expectancy
were determined.
According to the results of the study, positive dynamics of bee survival were observed for
10 days with 100 % in the III and IV experimental groups, against the background of lower
preservation for the II experimental group compared to the control. On day 10, the number of
live bees was lower and was 97.3 % in the second experimental group and remained at this
level for up to 20 days. After 30 days from the beginning of the experiment, the number of live
bees fluctuated, in particular in group II was 91.9 %; III – 97.7 %; IV – 97.9 % against control –
94.9 %. Not set bee death within 10 days in the first - control, III, and IV research groups. In
group II, 2.7 % of bee deaths were established, maintaining this value until the 20th day.
However, on the 30th day, there was an increase in mortality from 2.7 (20 days) to 8.1 % in the
second experimental group compared to the control. The third experimental group observed
lower levels of bee deaths within 30 days – 2.3 % against 5.1 % in the control group. The
number of dead bees in the experimental group IV at 30 days was 2.1 % and was lower than in
the control group during this period of the experiment. Life expectancy for bees III and IV
research groups was fed sugar syrup with the addition of Mg citrate at a dose of 0.02 mg and
0.01 Mg / l was 29.8 and 30.0 c.u. in accordance. Lower results in life expectancy (29.1) Bee
marked the second experimental group that received Mg citrate at a dose of 0.04 mg Mg / l
compared to 29.7 c.u. in control.
Therefore, a more pronounced positive effect of Mg citrate on the viability and resistance
of bees was observed for III and IV experimental groups, which received before feeding sugar
syrup citrate Mg in doses of 0.02 and 0.01 mg Mg / l.
Keywords: bees, citrate Mg, viability, resistance.

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5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and |82
Spiritual Human Development
November 3 2021
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