Page 85 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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DIVERSITY OF HIGH MOLECULAR WEIGHT GLUTENIN SUBUNIT ALLELES CONSERVED IN
THE EX-SITU COLLECTION OF AEGILOPS BIUNCIALIS VIS.
1,2
1,2
Natalia Kozub , Igor Sozinov , Hanna Bidnyk , Natalia Demianova ,
1,2
1
Oksana Sozinova , Yaroslav Blume
1,2
2
1 Institute of Plant Protection of the National Academy of Agrarian Sciences of Ukraine,
Kyiv, Ukraine; E-mail: natalkozub@gmail.com
2 Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine, Kyiv,
Ukraine
Aegilops biuncialis Vis. belongs to the tertiary gene pool of wheat and is a promising source
of new genes for wheat improvement. It is a tetraploid species with the genomic formula UUMM.
Ae. biuncialis is widespread in southeastern Europe, the Cis- and Transcaucasus regions, on the
western arc of the Fertile Crescent. The northern part of the Ae. biuncialis area resides in the
Crimean Peninsula. For enriching the common wheat gene pool, Ae. biuncialis may provide new
alleles for high molecular weight (HMW) glutenin subunits, which directly determine bread-
making quality. The study aimed to conserve ex situ the diversity of Ae. biuncialis, mainly from
populations from the Crimean Peninsula, concerning HMW glutenin subunit alleles.
Ae. biuncialis samples collected in different regions of the Crimea in 1995–2012 served as
the material for investigation. The samples represented 15 populations (5 from the western and
eastern regions of the Crimean Peninsula. 4 from the southern part, and 1 from the
southwestern region – from the Tauric Chersonese National Reserve). For propagation, single
spikes from natural populations were sown on the experimental plot (Kyiv region). SDS
electrophoresis according to Laemmli was performed to identify alleles at the Glu-U1 and Glu-
M 1 loci. The previously compiled catalogue of HMW glutenin subunits encoded by different
b
Glu-U1 and Glu-M 1 alleles was used for allele identification (Kozub et al., 2011). As a result of
b
analysis of 1200 Ae. biuncialis samples from the Crimean natural populations, this catalogue
was supplemented by new allelic variants. Samples with different storage protein alleles were
selected for further propagation. The collection was made in accordance with the M-strategy of
establishing a core collection (van Hintum et al., 2000).
In the current collection of the propagated 40 samples, there are 8 different alleles at the
locus Glu-U1 (a-g, and k) and 14 alleles at Glu-M 1 (a-i, m-o, q, and s). All these alleles at Glu-U1
b
encode two HMW glutenin subunits (both x- and y-subunits), whereas at Glu-M 1 five alleles are
b
encoding only one subunit: the alleles e, f, n, and s encode only the y-subunit and the allele Glu-
M 1i encodes only the x-subunit. The alleles encoding only one subunit are private alleles for a
b
Crimean population or a region. The collection diversity at the Glu-U1 and Glu-M 1 loci
b
represents 80 and 74 %, respectively, of the allele diversity, revealed among 1200 Ae. biuncialis
samples from the natural populations of the Crimean Peninsula. Of them, 24 samples have been
registered in the National Center of Plant Genetic Resources of Ukraine (NCPGRU, Kharkiv),
representing 60 and 47 % of the revealed genetic diversity at the Glu-U1 and Glu-M 1 loci,
b
respectively. Samples carrying seven new alleles have been propagated for further registration
in NCPGRU. The registered accessions of Ae. biuncialis are available for use in wide crosses with
wheat to transfer novel HMW glutenin subunits into the wheat gene pool. The geographical
differentiation of Ae. biuncialis by the flowering rate was revealed: the earliest are samples from
the eastern part of the area of the species in the Crimean Peninsula. Such samples are the most
convenient for interspecific hybridization with cultivated wheat.

Keywords: Aegilops biuncialis, high molecular weight glutenin subunits, alleles, bread-making quality.

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