Page 86 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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ANTIOXIDANT PROPERTIES OF DRY SUBSTANCE OF FLOWERS MEDICINAL PLANT
1
Mykhailo Kryvyi , Olena Dikhtiar , Dina Lisohurska , Jan Brindza
1
2
1
1 Polissya National University, Faculty of Technology, Zhytomyr, Ukraine;
E-mail.: olena.dikhtiar@gmail.com
2 Institute of Biological Conservation and Biosafety, Slovak University of Agriculture in Nitra, Slovak
Republic
The biological value of flowers of medicinal plants is determined by their total antioxidant
activity, which is due to the content of biologically active substances, phenolic compounds,
flavonoids, localized in various vegetative organs of plants.
Our research aimed to determine and compare the total antioxidant activity of methanol
and aqueous extracts of dry matter of flowers of medicinal plants for their further use in
beekeeping.
Plant raw materials of echinacea purpurea (Echinacea purpurea L.), oregano (Origanum
vulgare L.), sage (Salvia officinalis L.), tansy (Tanacetum vulgare L.), hyssop (Hyssopus officinalis
L.), tarragon (Artemisia dracunculus L.), wormwood. (Artemisia absinthium L.), veronica
longifolia (Veronica longifolia L.), сanadian goldenrod (Solidago canadensis L.), heather (Calluna
vulgaris (L.) Hull), which were used for analysis were obtained in conditions Polissya, Zhytomyr
region.
To date, several methods are known to assess the antioxidant potential of plants, one of
which is a spectrometric method with a stable chromogen radical DPPH (2,2-diphenyl-1-
picrylhydrazyl). According to the method, methanol and distilled water were used as
extractants. Before research, flowers with a dry matter content of 15–16 % were ground and
extracted in solvents. The solutions were constantly stirred on the shaker for 12 hours. We
added 3.9 ml of phenolic DPPH solution to the filtered extracts for further studies. We prepared
the solution for photometric studies immediately before the analysis. The antioxidant activity
of the dry matter of flowers was determined using a spectrophotometer Genesis-20. For
calculations, we measured the optical density of the radical solution and the optical density
with the sample 10 minutes after preparation. Experimental data were processed by the
method of mathematical statistics in Microsoft Excel, calculated the average value (M), its
standard error (m), and the coefficient of variation (V).
We found a high level of antioxidant action of plant extracts in methanol solution (71.1–
76.1 %); in particular, we found the highest potential is in Calluna vulgaris, Solidago canadensis,
Hyssopus officinalis, Tanacetum vulgare, Artemisia absinthium. In an aqueous solution, the
antioxidant effect of the extracts was in the range of 23.5–71.8 %. High values of antioxidant
capacity of aqueous extracts of flowers had Calluna vulgaris, Tanacetum vulgare, Veronica
longifolia. On the contrary, the lowest antioxidant capacity in aqueous extracts of Echinacea
purpurea, Origanum vulgare has been found. Coefficients of variation of antioxidant activity of
extracts of medicinal plants in methanol solutions range from 0.5 to 4.0 %, and in aqueous from
1.3 to 15.6 %. The most consolidated values of the coefficients of variation in methanol solutions
were obtained in Echinacea purpurea and Tanacetum vulgare, and in aquatic – Solidago
canadensis, Hyssopus officinalis.
The obtained data of studies of flowers of medicinal plant extracts testify to their
indisputable antioxidant properties. Therefore, they can potentially be used for bee colonies in
early spring.

Keywords: plant, extract, methanol, water, antioxidant activity.

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5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and |85
Spiritual Human Development
November 3 2021
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