Page 91 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

th

LOCALIZATION OF SAPROTROPHIC FUNGUS CHAETOMIUM COCHLIODES IN HEALTHY
TISSUES OF AGRICULTURAL PLANTS
Anna Kyslynska, Hanna Tsekhmister
Agricultural Microbiology and Agro-Industrial Production Institute of Agrarian Sciences National
Academy, Chernihiv, Ukraine; E-mail.: a.s.kyslynska@gmail.com
It is well-known that endophytic fungi can stay in plant tissues for a long time without any
sign of their presence and only be active under adverse environmental conditions. The study of
diversity, ecological niches, and metabolism products of endophytic microorganisms, their
usage to improve the growth and development of the plants, the macroorganism protection
from adverse environmental factors – all that are important aspects of applied microbiology in
agricultural production.
The study aimed to investigate the ability of Chaetomium cochliodes Palliser 3250 fungus
to penetrate healthy tissues of agricultural crop roots was determined.
Localization of the endophytic fungus mycelium within the macroorganism is revealed by
histological methods. Seeds of wheat, barley, rye, triticale, buckwheat, maize, sunflower,
soybean, and flax were inoculated with the saprotrophic fungus C. cochliodes and grown in the
vegetation experiment. C. cochliodes 3250 is a strain of the marsupial antagonist fungus that
was obtained from a collection of beneficial soil microorganisms from the Agricultural
Microbiology and Agro-Industrial Production Institute of Agrarian Sciences National Academy
of Ukraine Thin sections of the roots and plant root hairs were painted with an aniline blue lactic
acid solution, which allowed the visualization of fungal mycelium and, in some cases, spores.
As a result of microscopy of root hairs and roots cross-sections of the investigated plants,
it is established that the saprotrophic fungus C. cochliodes 3250 actively develops in the root
zone of the studied crops and forms fruiting bodies on the roots of wheat, rye, triticale, maize,
sunflower, soybean, and buckwheat. Fruit bodies of C. cochliodes 3250 formed mainly on the
secondary and tertiary lateral roots of plants. Some small hyphae were found inside the
rhizodermal cells, with larger hyphae being observed both between the rhizodermal cells and
in the spaces of the mesoderm parenchymal cells. The penetration of C. cochliodes 3250 hyphae
into the root and root hairs of the studied plants was also detected. Localization of fungus spores
on the plant's root hairs and samples with sections of mesoderm parenchymal cells is evidence
of endophytic associative system formation. Micromycetes penetration of C. cochliodes 3250 in
rhizoderm cells was recorded. It should be noted that C. cochliodes 3250 did not inhabit flax
roots. We did not observe any fruit bodies on the roots of this culture, nor hyphae penetration
inside the flax root hairs.
Thus, the fungus C. cochliodes 3250 forms fruiting bodies on the roots and penetrates the
root cells of wheat, barley, rye, triticale, buckwheat, corn, sunflower, and soybeans. Accordingly,
the localization of fungal structures in healthy root tissues of cereals, legumes, and industrial
crops indicated the ability of the saprotrophic fungus C. cochliodes 3250 to form endophytic
symbiotic systems.

Keywords: agricultural plants, Chaetomium cochliodes, fungal endophytes.
Acknowledgments
The work was performed within the state task of basic research No. 0116U003068.

th
5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and |90
Spiritual Human Development
November 3 2021
rd