Page 62 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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ANTIBACTERIAL ACTIVITY OF ETHANOLIC EXTRACTS OBTAINED FROM LEAVES OF
SOME THYMUS L. (LAMIACEAE MARTINOV) REPRESENTATIVES AGAINST
ESCHERICHIA COLI

Vitaliy Honcharenko , Viktor Nachychko , Andriy Prokopiv , Halyna Tkachenko ,
1
1,2
3
1,2
Natalia Kurhaluk , Anna Góralczyk
3
3
1 Ivan Franko National University of Lviv, Lviv, Ukraine;
E-mail.: vherbarium@ukr.net
2 Botanic Garden of Ivan Franko National University of Lviv, Lviv, Ukraine
3 Institute of Biology and Earth Sciences, Pomeranian University in Słupsk, Poland
The current study aimed to verify the antibacterial effects of four species and one
interspecific hybrid of the Thymus L. genus (Thymus serpyllum L. emend. Mill., T. pannonicus All.,
T. × porcii Borbás, T. pulegioides L., T. alpestris Tausch ex A. Kern.) sampled in the western part
of Ukraine against Escherichia coli (Migula) Castellani and Chalmers ATCC 25922™.
®
Leaves of T. serpyllum were collected among the grass on sandy soil in the edge of a pine
forest (Baymaky village, Bilohirya district, Khmelnytsky region, Ukraine; N 50°03´58,9´´, E
26°13´37,5´´, 257 m a.s.l.). Leaves of T. pannonicus were harvested among the grass on the
roadside between the two cultivated fields (Syvky village, Bilohirya district, Khmelnytsky
region, Ukraine; N 50°02´09,6´´, E 26°13´19,2´´, 283 m a.s.l.). Leaves of T. pulegioides were
collected among grass nearby land parcels (Syvky village, Bilohirya district, Khmelnytsky
region, Ukraine; N 50°02´02,8´´, E 26°14´13,9´´, 306 m a.s.l.). Leaves of Th.× porcii (a hybrid
between T. pannonicus and T. pulegioides) were sampled in the grass stand, on the side of the
footpath of the race track (Medovoi Pechery Str., Lviv, Ukraine; N 49˚49'15.1", E 24˚05'12.5",
348 m a.s.l.). Leaves of Th. alpestris were harvested on the side of the road below the stream, in
mountain valley Shumneska (Kvasy village, Rakhiv district, Zakarpattia region, Ukraine; N
48˚09'32.3", E 24˚21'26.4", 1259 m a.s.l.). Plant samples were thoroughly washed to remove all
the attached material and used to prepare ethanolic extracts. Fresh leaves were washed,
weighed, crushed, and homogenized in 96 % ethanol (in ratio 1:19, w/w) at room temperature.
Antimicrobial activity was determined using the agar disk diffusion technique. Isolates of
bacteria were then incubated at 37 C for 24 h. The plates were then observed for the zone of
0
inhibition produced by the antibacterial activity of various ethanolic extracts obtained from
leaves of Thymus representatives. Zone diameters were determined and averaged. Statistical
analysis of the data obtained was performed by employing the mean ± standard error of the
mean (S.E.M.).
The current study has shown that ethanolic extracts obtained from leaves of Thymus
species inhibited mild activity against E. coli. The mean diameter of inhibition zone for T.
serpyllum was (10.57 ±0.82 mm), for T. pannonicus – (11.59 ±0.75 mm), for T. x porcii – (10.80
±0.69 mm), for T. pulegioides – (10.15 ±0.71 mm), and for T. alpestris – (9.82 ±0.64 mm). The
highest antimicrobial effect was recorded for T. x porcii, T. pannonicus and T. serpyllum. It
should be noted that the most antimicrobial effective plant against E. coli was T. x porcii (mean
diameter of inhibition zone was 10.80 ±0.69 mm).
The biological activities of Thymus plants and essential oils depend on their chemical
composition, which is determined by the genotype and influenced by environmental conditions.
Nowadays, thymol and thyme present a wide range of functional possibilities in the pharmacy,
food, and cosmetic industry due to several studies that have evaluated the potential therapeutic
uses of this compound for the treatment of disorders affecting the respiratory, nervous, and
cardiovascular systems. Moreover, this compound also exhibits antimicrobial, antioxidant, anti-
carcinogenesis, anti-inflammatory, and antispasmodic activities, as well as a potential as a
growth enhancer and immunomodulator.
Keywords: Thymus species, leaves, antibacterial efficacy, disc diffusion technique, ethanolic extracts.

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Spiritual Human Development
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