Page 66 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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DEVELOPMENT OF THE ANALYTICAL PROCEDURE OF THE DETERMINATION OF TOTAL
FLAVONOID CONTENT IN THE HERB OF MONARDA FISTULOSA L.
Nataliia Hudz , Vladimira Horčinová-Sedláčková , Jana Šimková , Liudmyla Svydenko ,
2
2
1
3
Ján Brindza , Piotr Paweł Wieczorek
2
4
1 Department of Drug Technology and Biopharmacy, Danylo Halytsky Lviv National Medical University,
Lviv, Ukraine; E-mail.: natali_gudz@ukr.net
2 Institute of Biological and Environmental Sciences, Slovak University of Agriculture in Nitra, Slovakia
3 Sector of Mobilization and Conservation of Plant Resources of the Rice Institute of the NAAS, Plodove,
Ukraine
4 Department of Analytical Chemistry, University of Opole, Poland
Monarda fistulosa L. is to a promising new plant raw material. Essential oil of this species
exhibits bactericidal action against many microorganisms. The M. fistulosa herb contains
flavonoids of different groups (luteolin-7-O-glucoside, apigenin-7-O-glucoside, apigenin-7-O-
rutinoside, luteolin, apigenin, rutin, hesperidin, diosmin, quercetin, naringenin, catechin,
quercetin, etc.). According to Dmitrienko et al., the content of quercetin determined by high
pressure liquid chromatography is 0.6 mg per 1 g of the M. fistulosa leaves. The total flavonoid
content (TFC) in M. fistulosa herb is about 2 % and depends on a particle size of the herb,
ethanol concentration and place of growing. Therefore, this plant could be considered as a raw
material for the preparation of herbal products rich in flavonoids.
The herb of M. fistulosa was collected during the flowering period at the Sector of
Mobilization and Conservation of Plant Resources of the Rice Institute in 2020. Two tinctures
of the M. fistulosa herb were prepared by remaceration at the ratio of the herbal substance to a
final product was approximately 1 to 9 (tincture 1) and 1 to 4.5 (tincture 2) at a particle size of
0.5 to 3 mm (70 % ethanol). The TFC was evaluated by the developed analytical technique of
aluminum chloride method.
The analytical procedure of the determination of TFC was elaborated. 25 µL of the
developed tinctures were diluted with 50 % ethanol up to 1000 µL and were mixed with 1000
µL of 1.1 % solution of aluminum chloride. The mixtures were mixed. The incubation was at
room temperature for 85 ±10 min. 50 µL of the stock solution of rutin trihydrate (1032 mg/L)
or quercetin hydrate (400 mg/L) were diluted with 50 % ethanol up to 1000 µL. 1000 µL of 1.1
% aluminum chloride in 50 % ethanol were added to the obtained dilutions of rutin trihydrate
or quercetin hydrate and mixed. After incubation at room temperature for 85 ±10 min the
spectra of the reaction mixtures were measured in the range of 360 nm to 440 nm using the
spectrophotometer (Photometry Hitachi U-2810). The volume of 1.1% aluminum chloride in
50 % ethanol was substituted by the same volume of 50 % ethanol in the blanks for tinctures
and solutions of rutin trihydrate or quercetin hydrate. The results were expressed as mg eq-
rutin/L and mg eq-quercetin/L of a tincture and mg eq-rutin/g and mg eq-quercetin/g of the
M. fistulosa herb. The TFC was the following: tincture 1 – 1547.6 mg eq-rutin/L (14.3 mg eq-
rutin/g) and 563.3 mg eq-quercetin/L (5.2 mg eq-quercetin/g); tincture 2 – 1789.4 mg/L
(8.2 mg eq-rutin/g) and 584.3 mg eq-quercetin/L (2.7 mg eq-quercetin/g). These results point
to richness of the M. fistulosa herb in flavonoids. For instance, the TPC of the herbs of Satureja
montana and Satureja hortensis, grown on the same plots, ranged from 5.15 to 9.47 eq-rutin/g
and 6.67 to 7.98 eq-rutin/g, respectively.
The herb of M. fistulosa of Ukrainian origin is rich in flavonoids and could be used as a raw
material for the preparation of herbal preparations with antioxidant, antimicrobial,
immunostimulated properties.

Keywords: Monarda fistulosa, herb, total flavonoid content, flavonoids.
Acknowledgments
Co-authors thanks SAIA and Visegrad Fund for the scholarships for the research performed at the Slovak
University of Agriculture in Nitra (Slovakia), 2021.

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November 3 2021
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