Page 34 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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ASSESSMENT OF LIPID PEROXIDATION IN THE HUMAN BLOOD AFTER IN VITRO
TREATMENT BY LEAF EXTRACTS OBTAINED FROM SOME AGLAONEMA SPECIES
1
Lyudmyla Buyun , Halyna Tkachenko , Natalia Kurhaluk , Maryna Opryshko ,
2
2
1
Myroslava Maryniuk , Oleksandr Gyrenko
1
1
1 M.M. Gryshko National Botanic Garden, National Academy of Science of Ukraine, Kyiv, Ukraine;
E-mail.: buyun@nbg.kiev.ua
2 Institute of Biology and Earth Sciences, Pomeranian University in Słupsk, Poland
This study aimed to evaluate the antioxidant activity of water extracts obtained from
leaves of Aglaonema commutatum Schott, A. nitidum (Jack) Kunth, A. simplex (Blume) Blume,
and A. modestum Schott ex Engl. plants. The effect of the extract on cellular oxidative damage
was evaluated using in vitro bioassays by 2-thiobarbituric acid reactive substances (TBARS)
formation as a lipid peroxidation biomarker in human erythrocytes.
The leaves of Aglaonema plants, cultivated under glasshouse conditions, were sampled at
M.M. Gryshko National Botanical Garden (NBG), National Academy of Science of Ukraine (Kyiv,
Ukraine). Freshly collected leaves were washed, weighed, crushed, and homogenized in 0.1M
phosphate buffer (pH 7.4) (in proportion 1:19, w/w) at room temperature. The extracts were
then filtered and used for analysis. The extracts were stored at -20 °C until use. Blood (10–20
ml) was obtained from normal volunteers via venipuncture (4 males and 5 females aged 28–
53-years old). The Research Ethics Committee of Regional Medical Hospital in Gdańsk (Poland)
approved the study (KB-31/18). An erythrocyte suspension at 1 % hematocrit was incubated
with 4 mM phosphate buffer (pH 7.4) (control) and pre-incubated with the extracts (5 mg/mL)
at 37 °C for 60 min. This reaction mixture was shaken gently while being incubated for a fixed
interval at 37 °C. For positive control, phosphate buffer was used. Erythrocyte aliquots were
used in the study. The level of lipid peroxidation was determined by quantifying the
concentration of 2-thiobarbituric acid reacting substances (TBARS) with the Kamyshnikov
(2004) method for determining the malonic dialdehyde (MDA) concentration. The µmol of MDA
per L was calculated using 1.56·10 mM cm as the extinction coefficient. The mean ± S.E.M.
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values were calculated for each group to determine the significance of the intergroup
difference. The significance of differences between the values (significance level, p<0.05) was
examined using the Kruskal–Wallis H test with Statistica 8.0 software (StatSoft, Krakow,
Poland).
Our results revealed, that the treatment by extracts obtained from various plants of the
Aglaonema genus in a dose of 5 mg/mL increased the TBARS level when compared to untreated
erythrocytes. The most potent prooxidative effect was demonstrated by the A. nitidum and
A. commutatum compared to phosphate buffer as control samples (an increase of TBARS
content by 85.3 and 30.2 %, p<0.05, respectively). On the contrary, the minimum increase of
TBARS content in human erythrocyte suspensions was induced by A. simplex and A. modestum
extracts (by 42.8 and 35.2 %, p<0,05, respectively). In conclusion, the most potent prooxidative
effect was demonstrated by the A. nitidum and A. commutatum compared to phosphate buffer
as a control sample. The minimum increase of TBARS content in human erythrocyte suspension
was induced by A. modestum extract. Further research is needed to determine the effects of the
active compounds of various plants belonging to the Aglaonema genus on oxidative damage in
human erythrocytes.

Keywords: Aglaonema, leaves, lipid peroxidation, 2-thiobarbituric acid reactive substance,
erythrocytes.
Acknowledgments
This study was carried out during the Scholarship Program supported by The Visegrad Fund in the
Institute of Biology and Earth Sciences, Pomeranian University in Słupsk (Poland). We thank The
Visegrad Fund for supporting our study.

5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and |33
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Spiritual Human Development
November 3 2021
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