Page 37 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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INFLUENCE OF MANNOPROTEIN PREPARATION OBTAINED FROM THE MICROBIAL
WASTE OF THE BEER INDUSTRY ON TRITICALE SEEDS
Oleg Chiselitsa, Natalia Chiselitsa, Alina Beshliu, Nadejda Efremova,
Elena Tofan, Ana Lozan, Svetlana Boortseva, Maxim Byrsa
IP Institute of Microbiology and Biotechnology Chisinau, Republic of Moldova;
E-mail.: chiselita.natalia@gmail.com
Climate change, frequent droughts, abrupt changes in weather conditions during the
season, as well as in connection with this the growth of phytopathogenic diseases, have become
significant problems for the cultivation of grain crops. One of the methods that allow
withstanding adverse weather conditions is the treatment of seeds before planting. It is
directed not only at increasing and accelerating germination, but also at obtaining a high and
qualitative harvest.
Currently, various methods of seed processing are known using natural biologically active
microbial preparations, especially from yeasts. Results about the influence of preparations of
levurian origin on the indices of plant growth and development are described in the literature,
but specifically about the effect of mannoprotein preparations in this regard, data have not been
found.
In this context, the research aimed to test the mannoprotein preparation LB-MP in the
concentration of 10 mg/mL at the ability to stimulate seed germination, growth, and
development of Triticosecale Wittm plants, Ingen 40 variety.
The biologically active preparation based on yeast biomass from brewing waste from the
Kellers brewery, Budeshti was obtained through several stages: autolysis of yeast biomass with
sodium phosphate buffer, subsequent processing of the remaining biomass with 1N NaOH for
2 hours at a temperature of +80 ±5 °C, obtaining the alkaline extract and sedimenting the
mannoproteins with ethyl alcohol.
The seeds were treated with LB-MP mannoprotein preparation in the concentration of 10
mg/mL and its distilled water solutions of 0.33; 0.5; 1; 2 and 10 %/V. Distilled water was served
as a control. The experiments were performed at room temperature +23–24 °C, in three
repetitions. Three days after germination, the following parameters were evaluated: the formed
number of roots, the length of the roots and stem, the dry mass of the roots and stem of each
plantule.
The obtained results indicate that the treatment of seeds with LB-MP bio preparation in
the concentration of 10 mg/mL increases the number of roots by 6 %, the length of the roots
by 10 %, the dry mass of roots and stems by 4–11 % compared to the control sample.
The stimulating effect was also established in the case of seed processing with the
aqueous solution of the LB-MP preparation in the concentration of 0.5 %. The results indicate
a 10 % increase in the number of roots, a 5 % increase of stem length, a dry mass of roots and
stems by 6–10 %, compared to the control variant. The other concentrations of the preparation
had a neutral influence, the values of the studied indices oscillating at the control level or had a
negative effect.
Thus, taking into account the established effect and the fact that the preparation can be
obtained at a convenient price from the waste of the beer industry, research on the use of yeast
preparations as stimulants of crop plants is perspective.

Keywords: brewer's yeast, mannoproteins, stimulator, Triticosecale, seeds.
Acknowledgments
The results were obtained within Project 20.80009.5107.16. "New biologically active microbial
preparations for increasing the reproductive and productive potential of animals of zootechnical
interest", funded by ANCD.

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5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and |36
Spiritual Human Development
November 3 2021
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