Page 36 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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COMPARATIVE PHYTOCHEMICAL RESEARCH OF STELLARIA HOLOSTEA L. AND
STELLARIA MEDIA L.
Oksana Cherpak, Michael Cherpak
Danilo Halytsky Lviv National Medical University, Lviv, Ukraine; E-mail.: oksanacherpak@gmail.com

The aim of the study was a comparative phytochemical study of Stellaria holostea L. and
Stellaria media L. grass, in particular, qualitative and quantitative determination of
polyphenolic compounds, ascorbic acid, chlorophylls, and carotenoids, which show anti-
inflammatory, antimicrobial, antioxidant, antifungal, and reparative.
Objects – dried raw materials of Stellaria holostea L. and Stellaria media L. herb. Detection
of the main groups of biologically active substances of medicinal plant raw materials using
qualitative reactions is carried out by the generally accepted methods of the received water and
water-alcohol extracts. Quantitative determination of polyphenolic compounds included:
determination of the content of the sum of oxidizable phenols – in aqueous extracts and
determination of the content of flavonoids – in ethanolic extracts. Quantitative determination
of the content of the sum of oxidized phenols – titrimetric method. Quantitative content of
flavonoids was carried out by spectrophotometric method. Quantitative determination of
ascorbic acid in the studied samples is performed by titrimetric method with sodium 2,6-
dichlorodiphenolindophenolate according to the method presented in the SPU (State
Pharmacopoeia of Ukraine, 2001). Quantitative determination of carotenoid content was
determined by the spectrophotometric method.
The content of tannins, saponins, anthocyanins, and flavonoids was established by
phytochemical study of water and water-alcohol grass extracts of Stellaria holostea and
Stellaria media. Identification reactions and chromatography in a thin layer of sorbent revealed
the presence of ascorbic acid in the aqueous extracts of both study objects (Rf = 0.40–0.44). The
titrimetric method established the quantitative content of ascorbic acid in the grass of the
Stellaria media 48.25 mg%, which is 1.3 times higher than its content in the herb of the Stellaria
holostea 37.42 mg%. The quantitative content of the sum of oxidizable phenols in the Stellaria
holostea herb is 3.82 % slightly higher than the average Stellaria media herb 3.63 %. The
content of the amount of flavonoids in the Stellaria holostea herb was 0.88 %, which is 1.2 times
higher than their content in the Stellaria media herb 0.74 %. The content of Stellaria holostea
herb β-carotene 28 mg% is 1.3 times higher than their content in Stellaria media herb 21 mg%.
Stellaria holostea and Stellaria media herb are a source of polyphenolic compounds –
tannins and flavonoids, ascorbic acid and carotenoids, is a valuable medicinal plant raw
material for further use both independently and as a component in combined phytomedicines
with antimicrobials, antimicrobials, antifungal, hepatoprotective action.
Keywords: Stellaria holostea, Stellaria media, phytochemical research.

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5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and |35
Spiritual Human Development
November 3 2021
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