Page 27 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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COMPARISON OF TECHNOLOGICAL PARAMETERS OF IN VITRO GROWTH OF ARTEMISIA
VULGARIS L. AND CICHORIUM INTYBUS L. "HAIRY" ROOTS IN A MEDIUM WITH
DIFFERENT SUCROSE CONTENT

Taisa Bohdanovych, Nadiia Matvieieva
Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine, Kyiv,
Ukraine; E-mail.: bogdanovych_tais@ukr.net
Artemisia vulgaris L. and Cichorium intybus L. are herbaceous plants used for centuries in
traditional medicine and known for their antioxidant, hepatoprotective, antispasmolytic,
antibacterial, and antifungal effects. A. vulgaris was even called “the mother of herbs” for its
wide use, and C. intybus is a dietary supplement, coffee substitute, and sweetener. Those
properties are caused by the high content of bioactive secondary metabolites. To increase their
production in the plant, cocultivation with Agrobacterium rhizogenes bacteria is used. This
process leads to the formation of “hairy” roots with altered metabolism and thus boosted
production of bioactive substances.
The study aimed to investigate technological parameters of in vitro growth of “hairy” root
cultures in a liquid half-strength MS medium with different content of sucrose (20 and 40 g/l),
and also the analysis of the accumulation of flavonoids with antioxidant properties.
Determination of root mass gain overtime was done by direct weighing, after which extracts of
roots (in 70 % ethanol) were prepared and analyzed for flavonoid content by the standard
method with aluminum chloride.
The results of the study showed that the mass gain after 3 weeks of cultivation for A.
vulgaris was higher when cultivating in the liquid medium with 40 g/L of sucrose comparing to
20 g/L: 1.24 ±0.16 g comparing to 0.47 ±0.03 g. A similar effect was obtained in the case of C.
intybus cultivation: 24.34 ±2.88 g comparing to 20.40 ±2.71 g. The coefficient of the mass gain
(ratio of m2 to m0) for A. vulgaris was 4.98 (40 g/L of sucrose) and 2.11 (20 g/L of sucrose) and
for C. intybus correspondingly 52.54 and 40.22. Flavonoid content in the ethanol extract of A.
vulgaris after cultivation was 1.51 ±0.28 mg RE/g FW (20 g/L of sucrose). The total content of
flavonoids for the absolute mass increase was 1.36 mg RE/Δm (20 g/L of sucrose). The output
of flavonoids synthesis (how much flavonoids can be obtained from 1 g of initial mass) thus was
3.16 mg RE. In the case of C. intybus, flavonoid content in the ethanol extract after cultivation
was 1.07 ±0.24 mg RE/g FW (40 g/L of sucrose) and 0.65 ±0.14 mg RE/g FW (20 g/L of sucrose).
The total content of flavonoids for the absolute mass increase was 26.47 mg RE/Δm (40 g/L of
sucrose) and 13.51 mg RE/Δm (20 g/L of sucrose). The output of flavonoids synthesis thus was
56.32 mg RE and 26.47 mg RE correspondingly. As it is seen from the data, flavonoid content
per 1 g of fresh weight is slightly higher for A. vulgaris, but the mass growth rate is considerably
higher for C. intybus, which results in a much bigger output of secondary metabolites over time.
However, both species showed similarity in the growth rate increased with higher content of
sucrose in medium, which concludes that the most efficient cultivation of "hairy" roots of
common mugwort and chicory in terms of product output is with the content of sucrose 40 g/L.

Keywords: “hairy” roots, Artemisia vulgaris, Cichorium intybus, technological parameters, flavonoids.
Acknowledgments. This research was financially supported by the National Academy of Sciences of
Ukraine, grant No. 34 (II-2-21).

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