Page 155 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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CHARACTERISTICS OF THE STOMATAL APPARATUS OF THE LEAVES OF VARIETIES AND
CULTIVARS OF LYCIUM SPP.
Mykhailo Zhurba, Antonina Ilyinska, Ivan Gurnenko
M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine
Kyiv, Ukraine; E-mail.: zhurbamikhail@gmail.com
The stomata are sensitive to changes in ecological factors of the environment, which is
especially important with modern climate change. In current introduction studies, stomatal
morphometric markers are widely used to determine the adaptiveness of forms and cultivars
of different plant species.
We studied the size of stomata, the density of their distribution, as well as the
amphistomatic index of the leaves in 13 cultivars and varieties of Lycium barbarum L. (LB01,
LB02, LB03), L. chinense Mill. (Amber Sweet, Delikat, Q1, Tybet, Sweet Lifeberry) and L.
truncatum Y.C.Wang (LT01, N1 Lifeberry, Korean Big, New Big, Super Sweet), introduced in the
M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine.
The leaf surface was analyzed using a PEMMA-102 (SELMI) scanning electron microscope.
Fragments of leaf blades were dried, sprayed with copper, and the number of stomata per unit
area of the leaf was counted. The morphological types of stomata were determined according
to the classification of Baranova (1985), the size of the stomata was determined using the
AxioVision 4.8.2 software. The amphistomatic index of the leaves and the stomatal ratio were
determined according to Muir, 2018.
All studied cultivars and forms Lycium spp. have amphistomatic leaves. The stomata are
of the anomocytic type. The upper epidermis has fewer stomata compared to the lower.
Biometric analysis showed that there are significant differences in the number and size of
stomata in the studied plants. The dimensions of the stomata in the adaxial epidermis are from
23.37 to 33.57 μm in length and from 11.82 to 21.93 μm in width. The stomata of the abaxial
epidermis are generally somewhat larger, ranging in width from 25.16 to 32.11 µm and from
12.21 to 21.41 µm, respectively. The density of stomata on leaves varied significantly between
Lycium species. Their number per mm for the adaxial and abaxial surfaces of the leaf blade is
2
78.72–80.72 and 238.58–241.75 for L. barbarum, 12.15–26.07 and 98.09–123.69 for L. chinense,
52.95–78.12 and 79.86–105.90 for L. truncatum, respectively. The amphistomatic index of
Lycium leaves is L. barbarum 0.24–0.25; L. chinense 0.11–0.17; L. truncatum 0.34–0.45. The
stomatal ratio is: in L. barbarum – 0.33; in L. chinense – from 0.12 to 0.36; in L. truncatum – from
0.53 to 0.84.
According to the results of the study, the most adapted to the climatic conditions of the
forest-steppe and deserve further selection research are the cultivars N1 Lifeberry, Korean Big,
New Big, Super Sweet of the species L. truncatum.

Keywords: Lycium spp., cultivars, varieties, leaves, stomata, biometric analysis.

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5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and |154
Spiritual Human Development
November 3 2021
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