Page 156 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015
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MELISOPALINOLOGICAL ANALYSIS OF HONEY OBTAINED FROM NATURAL LAND OF
POLISYA OF UKRAINE

Oksana Sichenko , Olena Dikhtiar , Oleksandr Dmytrenko
1
2
1
1 Polissya National University, Faculty of Technology, Zhytomyr, Ukraine;
E-mail.: olena.dikhtiar@gmail.com

Melisopalinological, or pollen, analysis involves the identification and quantification of
pollen grains of different plant species by microscopic examination, expressed as a percentage.
The method of pollen analysis has become widespread in beekeeping and is used to establish
the botanical and geographical origin of honey and other bee products. Dominant, secondary,
insignificant pollen grains and their inclusion are determined in the pollen spectrum.
The purpose of our research is to determine the melisopalinological analysis of honey
obtained from natural phytocenosеs of Zhytomyr Polissya.
In the course of the analysis, samples of spring-summer and autumn periods of centrifugal
honey obtained in 2010 and 2020, which were selected on the territory of Zhytomyr forestry
"Pulinsky forestry of agro-industrial complex" s. Pokostivka.
The species composition of honey pollen grains was determined using a Leverhuk D320L
Didital microscope at a magnification of approx. 16х.об. 40., identifying the electronic global
database PalDat. According to the results of the species composition of pollen grains using a
method developed by the International Commission on Bee Biology at the International Union
of Biologocal Sciences (IUBS) determined the monoflora of honey.
Selected samples of honey in the spring-summer period of 2010 were characterized by
the dominance of pollen grains of Frangula alnus (34.8 %); in turn, pollen grains of Vaccinium
myrtillus (19.0 %), Epilobium angustifolium (14.0 %), Pulmonaria officinalis (12.6 %),
Vaccinium vitis-idaea (6.3%) belonged to the category of secondary pollen components;
insignificant elements included pollen grains Fragaria vesca (4.5%), Tilia cordata (4.4%), Salix
captea (3.1 %), Thymus serpyllum and Origanum vulgare (2.4 % each), Taraxacum officinale and
Robinia pseudoacacia (2.1 % each); and pollen grains Lamium album (1.8 %) and Geum rivale
(1.0 %) – to inclusions.
Honey samples, dated 2020, contained 34.9 % of the dominant pollen grains of Frangula
alnus; secondary elements included pollen components Vaccinium myrtillus (20.0 %),
Epilobium angustifolium (15.0 %), Pulmonaria officinalis (14.8 %), Vaccinium vitis-idaea
(7.1 %), Fragaria vesca (5.0 %); insignificant components included pollen grains of Tilia
cordata (4.5 %), Salix captea (3.8 %), Origanum vulgare (3.3 %), Thymus serpyllum (2.6 %) and
Taraxacum officinale (2.3 %); to inclusions – Robinia pseudoacacia (2.0 %), Lamium album and
Geum rivale (1.2 % each).
In the samples of autumn honey harvest, we recorded the dominance of Calluna vulgaris
– 59.6 % in 2010 and 60.2 % in 2020. Solidago virgaurea pollen grains belonged to the category
of secondary elements in 2010 (15.0 %) and 2020 (15.2 %).
Thus, honey samples of both years were characterized by pollen profiles of high similarity.
However, insignificant components of honey in 2010 – pollen grains Fragaria vesca and Robinia
pseudoacacia in 2020 belonged to the categories of secondary components and inclusions,
respectively.

Keywords: honey, pollen analysis, pollen grains, natural lands.

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