Page 154 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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AMINO ACID COMPOSITION OF LEAVES, FLOWERS, PEDICEL, FRUITS, AND SEEDS OF
SCHISANDRA CHINENSIS (TURCZ.) BAILL.
3
Mykhailo Zhurba , Natalia Hudz , Eva Ivanišová , Anna Adriana Bieniek ,
2
1
4
Agata Antoniewska
5
1 M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine, Kyiv,
Ukraine; E-mail.: zhurbamikhail@gmail.com
2 Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
3 Slovak University of Agriculture in Nitra, Slovak Republic
4 University of Warmia and Mazury in Olsztyn, Poland
5 Warsaw University of Life Sciences, Warsaw, Poland

Schisandra chinensis (Turcz.) Baill. (Chinese magnolia vine) a plant species well-known in
traditional Chinese Medicine and also in modern Chinese medicine. The fruits were used in the
treatment of the diseases of the gastrointestinal tract, respiratory failure, cardiovascular
diseases, in the states of body fatigue and weakness, excessive sweating. The S. chinensis is also
known from traditional Russian medicine as a tonic, reducing hunger, fatigue, delaying the aging
process, increasing vitality, and improving mental health. The fruits have hepatoprotective,
anti-inflammatory, anticancer, immunostimulant, anti-obesity, antiviral, antibacterial,
adaptogenic, ergogenic activity, antioxidative, and detoxification properties. Biologically active
compounds are located not only in fruits but in different parts of the plant: bark, shoots, leaves,
and seeds. There are practically no data on the biochemical composition and pharmacological
effects of S. chinensis flowers. This study aimed to investigate the qualitative and quantitive
content of amino acids of leaves, flowers, fruits, seeds, and pedicel of S. chinensis. Amino acids
were determined using post-column derivatisation with ninhydrin and a VIS detector by ion-
exchange liquid chromatography (Model AAA-400 amino acid analyzer, Ingos, Czech Republic).
Amino acid analysis has shown that the tested S. chinensis fruits, seeds, and pedicel
contained 18 amino acids (9 essential and 9 non-essential ones) whereas the leaves and flowers
contained 16 amino acids (8 essential and 8 non-essential). The total contents of amino acids,
essential amino acids, and non-essential amino acids of the flowers were significantly higher
than in the other part of the plant. The total amino acids in the leaves, flowers, fruits, pedicel,
and seeds of S. chinensis were 156.2, 197.1, 74.6, 37.2, and 122.5 g.kg , respectively. The highest
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glutamic acid content in flowers with the highest mean content among the tested amino acids,
accounting for more than 24 % of the entire amino acid profile. Aspartic acid and leucine were
the major amino acids detected in the leaves, and glutamic acid, arginine, and aspartic acid were
the main amino acids found in the seeds. Fruits and pedicel were found to have the lowest
amino acid content. The total non-essential amino acids in leaves, flowers, fruits, pedicel, and
seeds amounting to 87.4, 124.5, 48.2, 23.7, and 7.2 g.kg , respectively, and the percentage of
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total essential amino acids amounting to 55.95, 63.17, 64.61, 63.71, and 66.94 %, respectively.
Obtained results of this study indicated about high and diversified content of amino acids
and allow to suggest the availability of a wide specter of pharmacological activity of leaves,
flowers, fruits, pedicel, and seeds S. chinensis. The high content of amino acids in the flowers
indicates promising to use as a source of essential and non-essential amino acids, and can also
be used for in-depth study of other biologically active compounds.

Keywords: Schisandra chinensis, amino acids, leaves, flowers, fruits, pedicel, seeds.
Acknowledgments
The publication was prepared with the active participation of researchers in the International network
AgroBioNet and supported by the Visegrad Fund, Bilateral Scholarship of the Ministry of Education,
Science, Research and Sport, and SAIA (Slovak Republic).

5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and |153
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Spiritual Human Development
November 3 2021
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