Page 92 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

th

WASTES FROM VEGETABLE OIL PRODUCTION AS A SOURCE OF BIOACTIVE SUBSTANCES
Iryna Laguta , Oksana Stavynska , Pavlo Kuzema , Roman Ivannikov ,
1
1
2
1
Victor Anishchenko , Oksana Linnik
1
3
1 Chuiko Institute of Surface Chemistry of NAS of Ukraine, Kyiv, Ukraine
2 M.M. Gryshko National Botanic Garden of NAS of Ukraine, Kyiv, Ukraine
3 L.M. Litvinenko Institute of Physical-Organic and Coal Chemistry of NAS of Ukraine, Kyiv, Ukraine;
E-mail.: icvmtt34@gmail.com
Since the wastes from vegetable oil products contain a large amount of valuable
biologically active substances, the bioprocessing concept involving the consistent use of all
biomass components is one of the promising areas of research within the new European Union
initiative "European Green Course", which helps to protect the environment and to increase
human biosafety. Especially valuable are biowastes containing polyphenolic compounds with
antioxidant / reducing properties, which can be used, for example, in medicine (as
pharmacological agents with antioxidant and antimicrobial properties), in "green" synthesis of
metal nanoparticles (as reducing agents), in the energy industry (as stabilizers of biodiesel to
prevent its degradation during storage), etc.
This work aimed to investigate the composition and antioxidant properties of extracts
obtained from vegetable oil production biowastes. Pomaces from seeds of the grape, ryegrass,
flax, hemp, pumpkin, mustard, carduus marianus, and sunflower were selected for the study.
The extraction was performed in two stages: first, oil residues were removed from 10
grams of crushed pomace for 6 hours using hexane (500 ml); then extracts of phenolic
compounds were obtained by treatment with 96 % ethanol (500 ml) for 6 hours.
The composition of extracts was investigated using HPLC and MALDI MS methods while
antiradical activity was tested in the reactions with DPPH radicals; total polyphenol content
•
was determined using Folin-Ciocalteu assay. All the extracts were found to contain mostly
flavonoids (20 mg per 1 g of raw material), oxycinnamic acids and their derivatives
(17 mg/g); the basic aglycones of flavonoid derivatives were quercetin and kaempferol, and
oxycinnamic acids were represented mainly by coumaric, caffeic and sinapic acids. The results
of antioxidant studies have shown that six of the eight examined extracts demonstrate high
antiradical activity, inhibiting even in the diluted form up to 90 % of radicals. Pumpkin and flax
waste extracts are relatively less active, although even these extracts, diluted by 10 times,
inhibit about 20 and 50 % of DPPH radicals, respectively. The data on the antiradical activity of
the extracts correlate with the results of the Folin-Ciocalteu test: extracts from pumpkin and
flax waste are characterized by a total phenolic index of 0.5 and 1, while for other extracts this
index is 3–10.
The high content of antioxidants and high activity of extracts from vegetable oil
production wastes show promise for the use of waste materials to extract effective antioxidants
of natural origin.
Keywords: natural antioxidants, vegetable oil production wastes, antioxidant properties.

Acknowledgements
This work was financially supported by National Research Foundation of Ukraine (Project No
2020.01/0136 "Efficient use of renewable plant resources and photocatalytic conversion of biomass as
eco-innovative approaches for environmental protection and human biosafety").

th
5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and |91
Spiritual Human Development
November 3 2021
rd