Page 75 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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CONTENT OF MINERAL ELEMENTS IN TISSUES OF HONEY BEES UNDER FEEDING
CITRATE CO AND GE
2
Igor Kikish , Iryna Kovalchuk , Rostyslav Fedoruk
1
1
1 Institute of Animal Biology National Academy of Agrarian Sciences of Ukraine, Lviv, Ukraine;
E-mail.: irenakovalchuk@ukr.net
2 Lvivskyy National University of Veterinary Medicine and Biotechnology named S.Z. Hzhytskoho,
Lviv, Ukraine

The study of the biological effects of microelements on the honey bees colony and their
interaction with other elements in the application of mineral additives will allow finding
optimal and safe methods for stimulating the resistance of the organism of bees and their
productivity.
The research was conducted in the spring in a private apiary in the Lviv region on 4 groups
of bee families, three bee families in each. Control group (I) was fed with sugar syrup (300
ml/week/bee colony), experimental group II - under similar conditions received sugar syrup
with the addition of 30 μg of Co in the form of citrate, III experimental group - under similar
conditions received 60 μg of Ge and IV 30 μg Co citrate and 60 μg Ge citrate. The duration of the
watering citrate syrup and Co and Ge is 4 weeks. Trace elements were added to sugar syrup in
the form of citrates obtained from LLC "Nanomaterials and Nanotechnologies" in Kyiv and
made by the method of nanobiotechnology. To study tissue samples were taken specific
anatomical parts (head, chest, and abdomen) bee colonies from the control and experimental
groups. In samples of tissue homogenates of bees, the content of individual trace elements was
determined on an atomic absorption spectrophotometer SF-115PK. The digital data were
processed statistically using the computer program Microsoft EXCEL with the determination of the
mean values of M, their deviations ± m, and the degree of probability of intergroup differences using
the Student's ratio (p).
According to the results of the study, it was found that feeding with sugar syrup Co in the
form of citrate was characterized by an increase in Fe content in the homogenate of head tissues
of honey bees II and IV groups, as well as in thoracic tissues of bees, respectively 1.2 and 1.1
times (p < 0.05). The highest level of Cu (p <0.05) was observed in all tissues of bees of the
experimental groups, which indicates the possible greater assimilation of copper from the
syrup into the organisms of bees and their transformation into the tissues of these departments.
Significantly higher Zn content was observed in the head tissues in the II and III experimental
groups against the background of lower content in the IV (p <0.01) groups compared to the
control. The lowest level of this element was found in the tissues of the abdomen of bees II, III,
IV (p <0.05) compared to the experimental group control. The antagonistic effect of Co and Ge
citrates in metabolic processes on the accumulation of toxic metals such as Pb and Cd in bee
tissues has been established. Thus, the concentration of Pb decreased in the homogenate of
head and abdominal tissues of the II and III experimental groups, compared to the control.
However, it should be noted that the antagonistic effect on Pb and Cd was more pronounced in
the tissues of the head and abdomen of the IV experimental group.
Thus, experimental studies indicate the relationship between the content of Co and Ge in
the body of honey bees with other trace elements. The detected changes in the content of
microelements in the body of bees show tissue specificity and depend on the dose of feed
additives of citrate Co and Ge. Feeding of Co and Ge citrates with sugar syrup had a corrective
effect on the content of trace elements such as Fe, Cu, Zn, as well as Pb and Cd of the main,
thoracic and abdominal anatomical parts of honey bees, which indicates a dose-dependent
effect of Co and Ge citrates in metabolic processes of mineral nutrition of their body in the
spring. This allows us to assert power a decisive impact on honey bees optimization parameters
of mineral metabolism in different anatomical parts of the body of bees.
Keywords: bees, tissues of bees, head, chest, abdomen, citrate Co and Ge.

5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and |74
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Spiritual Human Development
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