Page 118 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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ANTIOXIDANT EFFECTS OF EXTRACTS DERIVED FROM ROOT AND STALK OF
CHELIDONIUM MAJUS L. COLLECTED FROM URBAN AND RURAL AREAS USING THE
MODEL OF EQUINE ERYTHROCYTES

Nataniel Stefanowski, Halyna Tkachenko, Natalia Kurhaluk
Institute of Biology and Earth Sciences, Pomeranian University in Słupsk, Poland;
E-mail.: natanielstefanowski89@gmail.com
Greater celandine (Chelidonium majus L., Papaveraceae) is a perennial herbaceous plant,
with an upright and spreading stem, large leaves, and yellow flowers collected on the tops of
the stems in rare umbel inflorescence. The main aim of the study was an assessment of the
oxidative stress biomarkers (2-thiobarbituric acid reactive substances (TBARS), carbonyl
derivatives content of protein oxidative modification (OMP), total antioxidant capacity (TAC))
and also activity of antioxidant enzymes (catalase, ceruloplasmin) in the equine plasma and
erythrocytes after treatment by extracts derived from roots and stalks of Chelidonium majus
collected from rural and urban agglomerations.
Plant materials were collected from natural habitats on the territory of the Kartuzy
district in the Pomeranian province (northern part of Poland). Freshly collected leaves were
washed, weighed, crushed, and homogenized in 0.1M phosphate buffer (pH 7.4) (in proportion
1:19, w/w). The equine plasma and erythrocyte aliquots were used in the study. A volume of
0.1 ml of the C. majus extract was added to 1.9 ml of clean equine erythrocytes or 1.9 mL of
plasma. After incubation of the mixture at 37 °C for 60 min with continuous stirring, samples
were used for the biochemical assays.
Our results demonstrated that statistically significant reductions in lipid peroxidation
byproducts (TBARS) were noted after incubation with root extracts of C. majus collected from
both urban (by 35 %, p<0.05) and rural (by 34 %, p<0.05) agglomerations compared to the
control samples. Stalk extracts from C. majus also reduced TBARS levels, but only extracts of C.
majus were collected from the rural areas; a statistically significant decrease by 21 % (p<0.05)
was observed compared to the control samples. The lowest values in the content of the
aldehyde derivatives of oxidatively modified proteins (OMP) were observed after incubation
with root extracts of C. majus collected from both rural and urban areas (by 4.9 and 7.5 %,
p<0.05). On the other hand, levels of ketonic derivatives of OMP were significantly increased
after incubation with stalk extracts of C. majus collected from both rural and urban areas (by
17 and 16.4 %, p<0.05) compared to the control samples, in contrast to root extracts of C. majus
collected from urban areas, where there was a statistically significant reduction in ketonic
derivatives of oxidatively modified proteins (by 15 %, p<0.05) compared to the control sample.
A significant increase in the total antioxidant capacity (TAC) levels were observed after
incubation with root and stalk extracts of C. majus collected from both urban and rural areas,
but the highest values were observed after incubation with root extracts collected from rural
areas (by 66.7 %, p<0.05) compared to the control samples. Stalk extracts of C. majus collected
from urban agglomerations were found to be most effective in increasing catalase activity (by
115 %, p<0.05). Both root and stalk extracts of C. majus collected from rural areas caused a
statistically significant reduction in ceruloplasmin levels (by 10 and 9 %, p<0.05, respectively).
These in vitro studies indicate that extracts from this plant are a significant source of
natural antioxidants that may help prevent the progression of various levels of oxidative stress.
However, the proportions of secondary metabolites responsible for the antioxidant activity of
C. majus extracts are currently unclear. Therefore, further studies are needed to isolate and
identify the antioxidant compounds present in the plant extracts.
Keywords: root and stalks extract, equine erythrocytes, plasma, lipid peroxidation, oxidatively modified
proteins, total antioxidant capacity.

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5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and |117
Spiritual Human Development
November 3 2021
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