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5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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ANTIBACTERIAL ACTIVITY OF ETHANOLIC EXTRACTS FROM AGLAONEMA
COMMUTATUM SCHOTT AND ITS CULTIVARS AGAINST ENTEROCOCCUS FAECALIS
Maryna Opryshko , Halyna Tkachenko , Lyudmyla Buyun ,Natalia Kurhaluk ,
1
2
2
1
Myroslava Maryniuk , Oleksandr Gyrenko
1
1
1 M.M. Gryshko National Botanic Garden, National Academy of Science of Ukraine, Kyiv, Ukraine;
E-mail.: maryna.opryshko@meta.ua
2 Institute of Biology and Earth Sciences, Pomeranian University in Słupsk, Poland
Aglaonema Schott (Araceae Juss.) is one of the most beautiful foliage plants, as are many
members of these monocotyledonous flowering plants in which flowers are borne on a type of
inflorescence called a spadix. Aglaonema includes many cultivars that are important
ornamental plants due to their tolerance to drought and low light and low relative humidity
levels encountered under interior conditions. Aglaonema plants have been widely used in
recent years because of their anti-aging and longevity properties, natural anti-allergic and anti-
inflammatory properties. The current study aimed to evaluate the antibacterial activity of
ethanolic extracts obtained from leaves of Aglaonema commutatum Schott and its cultivars
(Malay Beauty, Silver Queen, and Silver King), cultivated under glasshouse conditions at M.M.
Gryshko National Botanic Garden (NBG), National Academy of Science of Ukraine against
Enterococcus faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz ATCC 51299™ strain.
®
The leaves of A. commutatum and its cultivars, cultivated under glasshouse conditions,
were sampled at M.M. Gryshko National Botanic Garden (NBG), National Academy of Science of
Ukraine. Freshly sampled leaves were washed, weighed, and homogenized in 96 % ethanol (in
proportion 1:19) at room temperature. The extracts were then filtered and investigated for
their antimicrobial activity. The testing of the antibacterial activity of the plant extracts was
carried out in vitro by the Kirby-Bauer disc diffusion technique. The susceptibility of the test
organisms to the plant extracts was indicated by a clear zone of inhibition around the holes
containing the plant extracts and the diameter of the clear zone was taken as an indicator of
susceptibility. The following criteria for interpretation of inhibition zone diameter were used
to assign susceptibility or resistance of bacteria to the phytochemicals tested: Susceptible (S) ≥
15 mm, Intermediate (I) = 10–15 mm, and Resistant (R) ≤ 10 mm.
The ability of the selected ethanolic plant extracts obtained from leaves of A. commutatum
and its cultivars to inhibit E. faecalis growth was determined in this study. The results revealed
that four extracts exert antibacterial activity against this microorganism. However, the extracts
from variety A. commutatum and cultivar A. commutatum 'Silver Queen' exhibited higher
inhibitory activity than the extracts from cultivars Melay Beauty and Silver King. Maximum in
vitro inhibition was scored by 'Silver Queen', followed by variety A. commutatum, cultivars
Malay Beauty, and Silver King, which presented inhibition zones of (14.52 ±1.07) mm, (14.34
±0.98) mm, (13.71 ±1.10) mm, and (13.29 ±1.16) mm, respectively. In the case of the positive
controls, 96 % ethanol possesses a mild anti-C. freundii effect, which presented inhibition zones
of (9.15 ±0.99) mm. In conclusion, the ethanolic extracts obtained from the leaves of Aglaonema
commutatum and its cultivars (Malay Beauty, Silver Queen, and Silver King) has the potential
for use as natural antimicrobial agents. Further in vivo and in vitro antimicrobial, phytochemical
and toxicological studies are required to evaluate the chemotherapeutic effect of the plant.
Keywords: Aglaonema commutatum, leaves, ethanolic extract, antimicrobial activity, disk diffusion
method.
Acknowledgments
We are grateful to The Visegrad Fund for supporting our study.

5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and |105
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Spiritual Human Development
November 3 2021
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