Page 102 - Book of Abstracts

5 International Scientific Online Conference DOI: https://doi.org/10.15414/2021.9788055224015

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INVESTIGATION OF POLYPHENOL EXTRACT СOMPOSITION OF GREAT BURNET
(SANGUISORBA OFFICINALIS L.) FROM THE CARPATHIAN RESERVE FOR CREATION
PHYTOPOLYMER COATING

Anastasiia Mysholov, Olena Konovalova, Irina Hurtovenko, Oleg Gudzenko,
Nataliia Hudzenko
Private Higher Educational Establishment "Kyiv Medical University", Department of Pharmaceutical
and Biological Chemistry, Pharmacognosy;
Kyiv, Ukraine; E-mail.: a.mysholov.st@kmu.edu.ua

Nowadays the development of medicinal phytopolymer coatings of "phytocoating"
targeted action is a promising direction. From this point of view, the development of
phytopolymer coating with biologically active plant substance based on extract Great Burnet is
of scientific and practical interest.
This work aimed to analyze the qualitative and quantitative composition of polyphenol
compounds extracted from Great Burnet and their application in the creation of phytocoating.
The object of research: roots and rhizomes of Great Burnet (Sanguisorbae radix et
rhizoma), collected in the Carpathian Reserve in autumn 2018.
Identification of polyphenol compounds was carried out by chemical reactions, in
particular, flavonoids – by Bryant cyanidin reaction and detection of phenolic hydroxyl by
reaction with FeCl3; tanning agents – were detected by reaction with ammonium iron sulfate.
The phytopolymer coating was synthesized from polyethers and isocyanates in two stages.
The high-performance liquid chromatography (HPLC) method was used to determine the
content of flavonoids and catechins on Agilent Technologies 1200 liquid chromatography.
Acetonitrile (A) and 0.1 % water solution of formic acid (B) were used as the mobile phase for
flavonoids. Separation was carried out on the chromatographic column Zorbax SB-C18 (3.5 µm,
150 x 4.6 mm) (Agilent Technologies, USA), the flow rate through column 0.25 ml/min
temperature of thermostat 30 C, the volume of injection 4 µl. The detection was carried out
using a diode-matrix detector with a signal recording at 280 nm and a fixed absorption
spectrum at 210–700 nm. Methanol (A) and 0.1 % water solution of formic acid (B) was used as
the mobile phase for catechins. The detection was carried out using a diode-matrix detector
with a signal recording at 275 nm and a fixed absorption spectrum at 210–700 nm. The
quantitative content of the material under investigation was found to be dominated by
neogesperydin (112.17 mg/100 g), rutin (83.55 mg/100 g), and pyrotechquine (87.46 mg/100
g).
The extract of the Great Burnet was used as the carrier of medicinal substances for filling
in the synthesis of the phytopolymer coating. The hemostatic effect on the blood of the white
rats was investigated. The research was done with a microscope and a stopwatch. Blood
coagulation was observed after some time caused by phenol compounds released from
phytocoating.
Thus, the qualitative composition and quantitative content of flavonoids and catechins
have been investigated by the HPLC method. The predominant content of neogesperydin, rutin,
and pyrotechyn was established. The synthesized phytopolymer coating with the filling based
on Great Burnet extract allows suggesting that such coatings with hemostatic action will have
an important place in medicine. The obtained results testify to the perspectives of further
investigation of the hemostatic effect of the obtained "phytocoating" with extract of Sanguisorba
officinalis.
Keywords: Sanguisorba officinalis, flavonoids, high-performance liquid chromatography, hemostatic
effect, phytopolymer coating.

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5 International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and |101
Spiritual Human Development
November 3 2021
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